This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by May, J. S.
Right arrow Articles by Stevenson, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by May, J. S.
Right arrow Articles by Stevenson, P. G.

 Previous Article  |  Next Article 

Journal of Virology, March 2005, p. 3459-3467, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3459-3467.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Glycoprotein M Is an Essential Lytic Replication Protein of the Murine Gammaherpesvirus 68

Janet S. May, Susanna Colaco, and Philip G. Stevenson*

Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom

Received 28 July 2004/ Accepted 28 October 2004

All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (MHV-68). The mutant virus completely failed to propagate in normally permissive fibroblasts. The defective genome was rescued by either homologous recombination to restore the wild-type gM in situ or the insertion of an ectopic, intergenic expression cassette encoding gM into the viral genome. Thus, gM was essential for the lytic replication of MHV-68.

* Corresponding author. Mailing address: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Phone: 44-1223-336921. Fax: 44-1223-336926. E-mail:pgs27{at}mole.bio.cam.ac.uk.

Journal of Virology, March 2005, p. 3459-3467, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3459-3467.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gillet, L., Alenquer, M., Glauser, D. L., Colaco, S., May, J. S., Stevenson, P. G. (2009). Glycoprotein L sets the neutralization profile of murid herpesvirus 4. J. Gen. Virol. 90: 1202-1214 [Abstract] [Full Text]  
  • Gillet, L., May, J. S., Stevenson, P. G. (2009). In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection. J. Gen. Virol. 90: 602-613 [Abstract] [Full Text]  
  • Leege, T., Fuchs, W., Granzow, H., Kopp, M., Klupp, B. G., Mettenleiter, T. C. (2009). Effects of Simultaneous Deletion of pUL11 and Glycoprotein M on Virion Maturation of Herpes Simplex Virus Type 1. J. Virol. 83: 896-907 [Abstract] [Full Text]  
  • Gillet, L., Colaco, S., Stevenson, P. G. (2008). Glycoprotein B switches conformation during murid herpesvirus 4 entry. J. Gen. Virol. 89: 1352-1363 [Abstract] [Full Text]  
  • Gillet, L., Stevenson, P. G. (2007). Evidence for a Multiprotein Gamma-2 Herpesvirus Entry Complex. J. Virol. 81: 13082-13091 [Abstract] [Full Text]  
  • Gillet, L., May, J. S., Colaco, S., Stevenson, P. G. (2007). Glycoprotein L Disruption Reveals Two Functional Forms of the Murine Gammaherpesvirus 68 Glycoprotein H. J. Virol. 81: 280-291 [Abstract] [Full Text]  
  • Gillet, L., Gill, M. B., Colaco, S., Smith, C. M., Stevenson, P. G. (2006). Murine gammaherpesvirus-68 glycoprotein B presents a difficult neutralization target to monoclonal antibodies derived from infected mice. J. Gen. Virol. 87: 3515-3527 [Abstract] [Full Text]  
  • Gill, M. B., Gillet, L., Colaco, S., May, J. S., de Lima, B. D., Stevenson, P. G. (2006). Murine gammaherpesvirus-68 glycoprotein H-glycoprotein L complex is a major target for neutralizing monoclonal antibodies. J. Gen. Virol. 87: 1465-1475 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»