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Journal of Virology, March 2005, p. 3200-3205, Vol. 79, No. 5
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.5.3200-3205.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Entry of Pseudorabies Virus: an Immunogold-Labeling Study

Harald Granzow,1 Barbara G. Klupp,2 and Thomas C. Mettenleiter2*

Institutes of Infectology,1 Molecular Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany2

Received 9 August 2004/ Accepted 12 October 2004

Herpesviruses infect cells by fusion of the viral envelope with cellular membranes, primarily the plasma membrane. During this process structural components of the mature virion are lost from the invading nucleocapsid, which then travels along microtubules to the nuclear pore. We examined the penetration process by immunoelectron microscopy and analyzed which of the major tegument proteins remained associated with the incoming capsid. We show that the UL36, UL37, and US3 proteins were present at intracytoplasmic capsids after penetration, whereas the UL11, UL47, UL48, and UL49 tegument proteins were lost. Thus, the three capsid-associated tegument proteins are prime candidates for viral proteins that interact with cellular motor proteins for transport of nucleocapsids to the nucleus.

* Corresponding author. Mailing address: Friedrich-Loeffler-Institut, Boddenblick 5A, 17493 Greifswald-Insel Riems, Germany. Phone: 49-38351-7250. Fax: 49-38351-7151. E-mail: mettenleiter{at}rie.bfav.de.

Journal of Virology, March 2005, p. 3200-3205, Vol. 79, No. 5
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.5.3200-3205.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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