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Severe Acute Respiratory Syndrome Coronavirus 3a Protein Is a Viral Structural Protein

Naoto Ito,^1,2, Eric C. Mossel,^1, Krishna Narayanan,¹ Vsevolod L. Popov,³ Cheng Huang,¹ Taisuke Inoue,¹ Clarence J. Peters,^1,3 and Shinji Makino^1*

Departments of Microbiology and Immunology,¹ Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas,³ Laboratory of Zoonotic Diseases, Division of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, Gifu, Japan²

Received 4 August 2004/ Accepted 20 October 2004

The present study showed the association of a severe acute respiratory syndrome coronavirus (SCoV) accessory protein, 3a, with plasma membrane and intracellular SCoV particles in infected cells. 3a protein appeared to undergo posttranslational modifications in infected cells and was incorporated into SCoV particles, establishing that 3a protein was a SCoV structural protein.

N.I. and E.C.M. contributed equally to this study.

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