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Journal of Virology, February 2005, p. 2637-2642, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2637-2642.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of the Minimal Replicator of Kaposi's Sarcoma-Associated Herpesvirus Latent Origin

Jianhong Hu1,{dagger} and Rolf Renne1*

Division of Hematology/Oncology and Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio1

Received 26 May 2004/ Accepted 1 October 2004

The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) binds to two sites within the 801-bp-long terminal repeat (TR) and is the only viral protein required for episomal maintenance. While two or more copies of TR are required for long-term maintenance, a single TR confers LANA-dependent origin activity on plasmid DNA. Deletion mapping revealed a 71-bp-long minimal replicator containing two distinctive sequence elements: LANA binding sites (LBS1/2) and an adjacent 29- to 32-bp-long GC-rich sequence which we termed the replication element. Furthermore, the transcription factor Sp1 can bind to TR outside the minimal replicator and contributes to TR's previously reported enhancer activity.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, Shands Cancer Center, University of Florida, Gainesville, FL 32610-0232. Phone: (352) 392-9848. Fax: (352) 392-5802. E-mail: rrenne{at}ufscc.ufl.edu.

{dagger} Present address: Department of Molecular Genetics and Microbiology, Shands Cancer Center, University of Florida, Gainesville, FL 32610-0232.

Journal of Virology, February 2005, p. 2637-2642, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2637-2642.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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