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Journal of Virology, February 2005, p. 2626-2630, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2626-2630.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Increased Efficiency of Phorbol Ester-Induced Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus during S Phase

Shane C. McAllister,1 Scott G. Hansen,1 Ilhem Messaoudi,1 Janko Nikolich-Zugich,1 and Ashlee V. Moses1*

Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon1

Received 12 May 2004/ Accepted 1 October 2004

Expression of Kaposi's sarcoma-associated herpesvirus (KSHV) lytic genes is thought to be essential for the establishment and progression of KSHV-induced diseases. The inefficiency of lytic reactivation in various in vitro systems hampers the study of lytic genes in the context of whole virus. We report here increased expression of KSHV lytic genes and increased release of progeny virus when synchronized cultures of body cavity-based lymphoma-1 cells are treated with a phorbol ester during S phase of the cell cycle.

* Corresponding author. Mailing address: Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505 NW 185th Ave., Beaverton, OR 97006. Phone: (503) 418-2712. Fax: (503) 418-2713. E-mail: mosesa{at}ohsu.edu.

Journal of Virology, February 2005, p. 2626-2630, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2626-2630.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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