This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xue, Y.
Right arrow Articles by Engel, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xue, Y.
Right arrow Articles by Engel, D. A.

 Previous Article  |  Next Article 

Journal of Virology, February 2005, p. 2474-2483, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2474-2483.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Adenovirus Protein VII Functions throughout Early Phase and Interacts with Cellular Proteins SET and pp32

Yuming Xue,1,{dagger} Jeffrey S. Johnson,1,{dagger} David A. Ornelles,2 Judy Lieberman,3 and Daniel A. Engel1*

Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia,1 Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina,2 CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts3

Received 12 August 2004/ Accepted 5 October 2004

Adenovirus protein VII is the major component of the viral nucleoprotein core. It is a highly basic nonspecific DNA-binding protein that condenses viral DNA inside the capsid. We have investigated the fate and function of protein VII during infection. "Input" protein VII persisted in the nucleus throughout early phase and the beginning of DNA replication. Chromatin immunoprecipitation revealed that input protein VII remained associated with viral DNA during this period. Two cellular proteins, SET and pp32, also associated with viral DNA during early phase. They are components of two multiprotein complexes, the SET and INHAT complexes, implicated in chromatin-related activities. Protein VII associated with SET and pp32 in vitro and distinct domains of protein VII were responsible for binding to the two proteins. Interestingly, protein VII was found in novel nuclear dot structures as visualized by immunofluorescence. The dots likely represent individual infectious genomes in association with protein VII. They appeared within 30 min after infection and localized in the nucleus with a peak of intensity between 4 and 10 h postinfection. After this, their intensity decreased and they disappeared between 16 and 24 h postinfection. Interestingly, disappearance of the dots required ongoing RNA synthesis but not DNA synthesis. Taken together these data indicate that protein VII has an ongoing role during early phase and the beginning of DNA replication.

* Corresponding author. Mailing address: Department of Microbiology, University of Virginia Health System, P.O. Box 800734, Charlottesville, VA 22908. Phone: (434) 924-8633. Fax: (434) 982-1071. E-mail: dae2s{at}virginia.edu.

{dagger} Y.X. and J.S.J. contributed equally to this work.

Journal of Virology, February 2005, p. 2474-2483, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2474-2483.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Russell, W. C. (2009). Adenoviruses: update on structure and function. J. Gen. Virol. 90: 1-20 [Abstract] [Full Text]  
  • Ali, H., LeRoy, G., Bridge, G., Flint, S. J. (2007). The Adenovirus L4 33-Kilodalton Protein Binds to Intragenic Sequences of the Major Late Promoter Required for Late Phase-Specific Stimulation of Transcription. J. Virol. 81: 1327-1338 [Abstract] [Full Text]  
  • Pegoraro, G., Marcello, A., Myers, M. P., Giacca, M. (2006). Regulation of Adeno-Associated Virus DNA Replication by the Cellular TAF-I/Set Complex. J. Virol. 80: 6855-6864 [Abstract] [Full Text]  
  • Haruki, H., Okuwaki, M., Miyagishi, M., Taira, K., Nagata, K. (2006). Involvement of Template-Activating Factor I/SET in Transcription of Adenovirus Early Genes as a Positive-Acting Factor. J. Virol. 80: 794-801 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»