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Journal of Virology, February 2005, p. 1966-1969, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1966-1969.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nitric Oxide Inhibits the Replication Cycle of Severe Acute Respiratory Syndrome Coronavirus

Sara Åkerström,1 Mehrdad Mousavi-Jazi,2 Jonas Klingström,1,3 Mikael Leijon,2 Åke Lundkvist,1,3 and Ali Mirazimi1*

Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, Solna,1 LightUp Technologies, Huddinge,2 MTC/Karolinska Institutet, Stockholm, Sweden3

Received 13 May 2004/ Accepted 16 September 2004

Nitric oxide (NO) is an important signaling molecule between cells which has been shown to have an inhibitory effect on some virus infections. The purpose of this study was to examine whether NO inhibits the replication cycle of the severe acute respiratory syndrome coronavirus (SARS CoV) in vitro. We found that an organic NO donor, S-nitroso-N-acetylpenicillamine, significantly inhibited the replication cycle of SARS CoV in a concentration-dependent manner. We also show here that NO inhibits viral protein and RNA synthesis. Furthermore, we demonstrate that NO generated by inducible nitric oxide synthase, an enzyme that produces NO, inhibits the SARS CoV replication cycle.

* Corresponding author. Mailing address: Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden. Phone: 46 (8) 457 25 73. Fax: 46 (8) 30 79 57. E-mail: Ali.Mirazimi{at}smi.ki.se.

Journal of Virology, February 2005, p. 1966-1969, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1966-1969.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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