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Journal of Virology, February 2005, p. 1961-1965, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1961-1965.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Human Herpesvirus 6B Induces Cell Cycle Arrest Concomitant with p53 Phosphorylation and Accumulation in T Cells

Bodil Øster, Bettina Bundgaard, and Per Höllsberg*

Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark

Received 25 March 2004/ Accepted 7 September 2004

We studied the interactions between human herpesvirus 6B (HHV-6B) and its host cell. Productive infections of T-cell lines led to G1/S- and G2/M-phase arrest in the cell cycle concomitant with an increased level and enhanced DNA-binding activity of p53. More than 70% of HHV-6B-infected cells did not bind annexin V, indicating that the majority of cells were not undergoing apoptosis. HHV-6B infection induced Ser20 and Ser15 phosphorylation on p53, and the latter was inhibited by caffeine, an ataxia telangiectasia mutated kinase inhibitor. Thus, a productive HHV-6B infection suppresses T-cell proliferation concomitant with the phosphorylation and accumulation of p53.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark. Phone: 45 8942 1772. Fax: 45 8619 6128. E-mail: ph{at}microbiology.au.dk.

Journal of Virology, February 2005, p. 1961-1965, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1961-1965.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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