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Journal of Virology, February 2005, p. 1906-1910, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1906-1910.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Two Neutralizing Regions on the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Produced from the Mammalian Expression System

Shixia Wang,{dagger} Te-hui W. Chou,{dagger} Pavlo V. Sakhatskyy, Song Huang, John M. Lawrence, Hong Cao, Xiaoyun Huang, and Shan Lu*

Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts

Received 7 July 2004/ Accepted 13 September 2004

The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).

* Corresponding author. Mailing address: Department of Medicine, University of Massachusetts Medical School, 364 Plantation St., Lazare Research Building, Worcester, MA 01605-2397. Phone: (508) 856-6791. Fax: (508) 856-6751. E-mail: shan.lu{at}umassmed.edu.

{dagger} S.W. and T.-h.W.C. contributed equally to this study.

Journal of Virology, February 2005, p. 1906-1910, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1906-1910.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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