This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pillai, S. K.
Right arrow Articles by Smith, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pillai, S. K.
Right arrow Articles by Smith, D. M.

 Previous Article  |  Next Article 

Journal of Virology, February 2005, p. 1734-1742, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1734-1742.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Semen-Specific Genetic Characteristics of Human Immunodeficiency Virus Type 1 env{dagger}

Satish K. Pillai,1* Benjamin Good,2 Sergei Kosakovsky Pond,1 Joseph K. Wong,1,2 Matt C. Strain,1 Douglas D. Richman,1,2 and Davey M. Smith1

University of California, San Diego, La Jolla,1 Veterans Administration, San Diego Healthcare System, San Diego, California2

Received 2 July 2004/ Accepted 10 September 2004

Human immunodeficiency virus type 1 (HIV-1) in the male genital tract may comprise virus produced locally in addition to virus transported from the circulation. Virus produced in the male genital tract may be genetically distinct, due to tissue-specific cellular characteristics and immunological pressures. HIV-1 env sequences derived from paired blood and semen samples from the Los Alamos HIV Sequence Database were analyzed to ascertain a male genital tract-specific viral signature. Machine learning algorithms could predict seminal tropism based on env sequences with accuracies exceeding 90%, suggesting that a strong genetic signature does exist for virus replicating in the male genital tract. Additionally, semen-derived viral populations exhibited constrained diversity (P < 0.05), decreased levels of positive selection (P < 0.025), decreased CXCR4 coreceptor utilization, and altered glycosylation patterns. Our analysis suggests that the male genital tract represents a distinct selective environment that contributes to the apparent genetic bottlenecks associated with the sexual transmission of HIV-1.

* Corresponding author. Mailing address: University of California, San Diego, Division of Biological Sciences, 9500 Gilman Dr., MC 0679, La Jolla, CA 92093. Phone: (858) 552-8585, ext. 7169. Fax: (858) 552-7445. E-mail: satish{at}ucsd.edu.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, February 2005, p. 1734-1742, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1734-1742.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bull, M. E., Learn, G. H., McElhone, S., Hitti, J., Lockhart, D., Holte, S., Dragavon, J., Coombs, R. W., Mullins, J. I., Frenkel, L. M. (2009). Monotypic Human Immunodeficiency Virus Type 1 Genotypes across the Uterine Cervix and in Blood Suggest Proliferation of Cells with Provirus. J. Virol. 83: 6020-6028 [Abstract] [Full Text]  
  • Huyser, C. (2008). Affordable ART services in Africa: synthesis and adaptation of laboratory services. ESHRE Monogr 2008: 77-84 [Abstract] [Full Text]  
  • Zarate, S., Pond, S. L. K., Shapshak, P., Frost, S. D. W. (2007). Comparative Study of Methods for Detecting Sequence Compartmentalization in Human Immunodeficiency Virus Type 1. J. Virol. 81: 6643-6651 [Abstract] [Full Text]  
  • Noviello, C. M., Pond, S. L. K., Lewis, M. J., Richman, D. D., Pillai, S. K., Yang, O. O., Little, S. J., Smith, D. M., Guatelli, J. C. (2007). Maintenance of Nef-Mediated Modulation of Major Histocompatibility Complex Class I and CD4 after Sexual Transmission of Human Immunodeficiency Virus Type 1. J. Virol. 81: 4776-4786 [Abstract] [Full Text]  
  • Pillai, S. K., Pond, S. L. K., Liu, Y., Good, B. M., Strain, M. C., Ellis, R. J., Letendre, S., Smith, D. M., Gunthard, H. F., Grant, I., Marcotte, T. D., McCutchan, J. A., Richman, D. D., Wong, J. K. (2006). Genetic attributes of cerebrospinal fluid-derived HIV-1 env. Brain 129: 1872-1883 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»