This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hatziioannou, T.
Right arrow Articles by Bieniasz, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hatziioannou, T.
Right arrow Articles by Bieniasz, P. D.

 Previous Article  |  Next Article 

Journal of Virology, December 2005, p. 15586-15589, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15586-15589.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Matrix-Induced Inhibition of Membrane Binding Contributes to Human Immunodeficiency Virus Type 1 Particle Assembly Defects in Murine Cells

Theodora Hatziioannou, Juan Martin-Serrano,{dagger} Trinity Zang, and Paul D. Bieniasz*

Aaron Diamond AIDS Research Center and the Rockefeller University, New York, New York

Received 1 August 2005/ Accepted 20 September 2005

Defective human immunodeficiency virus type 1 (HIV-1) assembly in murine cells is accompanied by poor plasma membrane binding and proteolytic processing of the HIV-1 Gag precursor. Here, we show that such defects are induced by the propensity of the HIV-1 MA globular head to inhibit membrane binding and particle assembly, particularly at the low expression levels observed in murine cells. Simple additions to or deletion of the MA globular head can improve the yield of infectious virions from murine cells by >50-fold. Expression level and autoinhibition can be important confounding variables in studies of HIV-1 assembly and contribute to defects encountered in murine cells.

* Corresponding author. Mailing address: Aaron Diamond AIDS Research Center, 455 First Avenue, New York, NY 10016. Phone: (212) 448-5070. Fax: (212) 725-1126. E-mail: pbienias{at}adarc.org.

{dagger} Present address: Department of Infectious Diseases GKT School of Medicine, King's College London, 2nd Floor, New Guy's House, Guy's Hospital, London SE1 9RT, United Kingdom.

Journal of Virology, December 2005, p. 15586-15589, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15586-15589.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sherer, N. M., Swanson, C. M., Papaioannou, S., Malim, M. H. (2009). Matrix Mediates the Functional Link between Human Immunodeficiency Virus Type 1 RNA Nuclear Export Elements and the Assembly Competency of Gag in Murine Cells. J. Virol. 83: 8525-8535 [Abstract] [Full Text]  
  • Coskun, A. K., van Maanen, M., Nguyen, V., Sutton, R. E. (2006). Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1.. J. Virol. 80: 3406-3415 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»