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Tat_28-35SL8-Specific CD8⁺ T Lymphocytes Are More Effective than Gag_181-189CM9-Specific CD8⁺ T Lymphocytes at Suppressing Simian Immunodeficiency Virus Replication in a Functional In Vitro Assay

Wisconsin National Primate Research Center,¹ Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin 53715²

Received 14 July 2005/ Accepted 3 September 2005

Epitope-specific CD8⁺ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8⁺ T lymphocytes. Certain epitope-specific CD8⁺ T lymphocytes may be better than others at suppressing viral replication. We compared the antiviral efficacy of two immunodominant CD8⁺ T lymphocyte responses—Tat_28-35SL8 and Gag_181-189CM9—by using a functional in vitro assay. Viral suppression by Tat-specific CD8⁺ T lymphocytes was consistently greater than that of Gag-specific CD8⁺ T lymphocytes. Such differences in antigen-specific CD8⁺-T-lymphocyte efficacy may be important for selecting CD8⁺ T lymphocyte epitopes for inclusion in future HIV vaccines.

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