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Journal of Virology, November 2005, p. 14429-14436, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14429-14436.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

CD46 Is a Cellular Receptor for All Species B Adenoviruses except Types 3 and 7

Marko Marttila,1 David Persson,1 Dan Gustafsson,1 M. Kathryn Liszewski,2 John P. Atkinson,2 Göran Wadell,1 and Niklas Arnberg1*

Department of Virology, Umeå University, SE-901 85 Umeå, Sweden,1 Department of Medicine, Division of Rheumatology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, Missouri 631102

Received 28 June 2005/ Accepted 17 August 2005

The 51 human adenovirus serotypes are divided into six species (A to F). Adenovirus serotypes from all species except species B utilize the coxsackie-adenovirus receptor for attachment to host cells in vitro. Species B adenoviruses primarily cause ocular and respiratory tract infections, but certain serotypes are also associated with renal disease. We have previously demonstrated that adenovirus type 11 (species B) uses CD46 (membrane cofactor protein) as a cellular receptor instead of the coxsackie-adenovirus receptor (A. Segerman et al., J. Virol. 77:9183-9191, 2003). In the present study, we found that transfection with human CD46 cDNA rendered poorly permissive Chinese hamster ovary cells more permissive to infection by all species B adenovirus serotypes except adenovirus types 3 and 7. Moreover, rabbit antiserum against human CD46 blocked or efficiently inhibited all species B serotypes except adenovirus types 3 and 7 from infecting human A549 cells. We also sequenced the gene encoding the fiber protein of adenovirus type 50 (species B) and compared it with the corresponding amino acid sequences from selected serotypes, including all other serotypes of species B. From the results obtained, we conclude that CD46 is a major cellular receptor on A549 cells for all species B adenoviruses except types 3 and 7.

* Corresponding author. Mailing address: Department of Virology, Umeå University, SE-901 85 Umeå, Sweden. Phone: 46 90 785 8440. Fax: 46 90 129905. E-mail: niklas.arnberg{at}climi.umu.se.

Journal of Virology, November 2005, p. 14429-14436, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14429-14436.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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