This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mustafa, F. Articles by Rizvi, T. A. Search for Related Content PubMed PubMed Citation Articles by Mustafa, F. Articles by Rizvi, T. A.

Sequences Intervening between the Core Packaging Determinants Are Dispensable for Maintaining the Packaging Potential and Propagation of Feline Immunodeficiency Virus Transfer Vector RNAs

Departments of Biochemistry,¹ Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates²

Received 23 May 2005/ Accepted 4 August 2005

The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to 150 bp of the 5' untranslated region and the first 100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.

This article has been cited by other articles:

• James, L., Sargueil, B. (2008). RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region. Nucleic Acids Res 0: gkn447v1-gkn447 [Abstract] [Full Text]