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Journal of Virology, November 2005, p. 13817-13821, Vol. 79, No. 21
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.21.13817-13821.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sequences Intervening between the Core Packaging Determinants Are Dispensable for Maintaining the Packaging Potential and Propagation of Feline Immunodeficiency Virus Transfer Vector RNAs

Farah Mustafa,1 Akela Ghazawi,2 Preethi Jayanth,2 Pretty Susan Phillip,2 Jahabar Ali,2 and Tahir A. Rizvi2*

Departments of Biochemistry,1 Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates2

Received 23 May 2005/ Accepted 4 August 2005

The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to ~150 bp of the 5' untranslated region and the first ~100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.

* Corresponding author. Mailing address: Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates. Phone: (971) 3-713-7514. Fax: (971) 3-767-1966. E-mail: tarizvi{at}uaeu.ac.ae.

Journal of Virology, November 2005, p. 13817-13821, Vol. 79, No. 21
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.21.13817-13821.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kenyon, J. C., Ghazawi, A., Cheung, W. K.S., Phillip, P. S., Rizvi, T. A., Lever, A. M.L. (2008). The secondary structure of the 5' end of the FIV genome reveals a long-range interaction between R/U5 and gag sequences, and a large, stable stem-loop. RNA 14: 2597-2608 [Abstract] [Full Text]  
  • James, L., Sargueil, B. (2008). RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region. Nucleic Acids Res 36: 4653-4666 [Abstract] [Full Text]  


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