This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oh, J. Articles by Broyles, S. S. Search for Related Content PubMed PubMed Citation Articles by Oh, J. Articles by Broyles, S. S.

 Previous Article  |  Next Article 

Journal of Virology, October 2005, p. 12852-12860, Vol. 79, No. 20
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.20.12852-12860.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Host Cell Nuclear Proteins Are Recruited to Cytoplasmic Vaccinia Virus Replication Complexes

Jaewook Oh and Steven S. Broyles^*

Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907

Received 13 October 2004/ Accepted 1 August 2005

The initiation and termination of vaccinia virus postreplicative transcription have been reported to require cellular proteins, some of which are believed to be nuclear proteins. Vaccinia virus replicates in the cytoplasmic compartment of the cell, raising questions as to whether vaccinia virus has access to nuclear proteins. This was addressed here by following the fate of several nuclear proteins after infection of cells with vaccinia virus. The nuclear transcription factors YY1, SP1, and TATA binding protein were found to colocalize with virus replication complexes in the cytoplasm of infected cells. In addition, the nuclear proteins RNA polymerase II, TAFIIp32, and histone deacetylase 8, but not the structural protein lamin B, also were found in the cytoplasm of the cell. The association of YY1 with replication complexes was dependent on DNA replication and required only the DNA binding domain of the protein, indicating that DNA binding alone may be responsible for the association of nuclear transcription factors with viral replication complexes in the cytoplasm. The cytoplasmic localization of YY1 was resistant to the nuclear export inhibitor leptomycin B. Evidence is presented indicating that nuclear import and export pathways were not adversely affected by vaccinia virus infection. These observations indicate that vaccinia virus replication complexes have ready access to nuclear proteins by allowing leakage from the nucleus.

Present address: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6076.

This article has been cited by other articles:

• Lin, Y.-C. J., Li, J., Irwin, C. R., Jenkins, H., DeLange, L., Evans, D. H. (2008). Vaccinia Virus DNA Ligase Recruits Cellular Topoisomerase II to Sites of Viral Replication and Assembly. J. Virol. 82: 5922-5932 [Abstract] [Full Text]  
• Hakki, M., Marshall, E. E., De Niro, K. L., Geballe, A. P. (2006). Binding and Nuclear Relocalization of Protein Kinase R by Human Cytomegalovirus TRS1. J. Virol. 80: 11817-11826 [Abstract] [Full Text]  
• Chahroudi, A., Garber, D. A., Reeves, P., Liu, L., Kalman, D., Feinberg, M. B. (2006). Differences and similarities in viral life cycle progression and host cell physiology after infection of human dendritic cells with modified vaccinia virus ankara and vaccinia virus.. J. Virol. 80: 8469-8481 [Abstract] [Full Text]  
• Hsiao, J.-C., Chao, C.-C., Young, M.-J., Chang, Y.-T., Cho, E.-C., Chang, W. (2006). A Poxvirus Host Range Protein, CP77, Binds to a Cellular Protein, HMG20A, and Regulates Its Dissociation from the Vaccinia Virus Genome in CHO-K1 Cells.. J. Virol. 80: 7714-7728 [Abstract] [Full Text]  
• Knutson, B. A., Liu, X., Oh, J., Broyles, S. S. (2006). Vaccinia Virus Intermediate and Late Promoter Elements Are Targeted by the TATA-Binding Protein. J. Virol. 80: 6784-6793 [Abstract] [Full Text]