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Journal of Virology, January 2005, p. 677-683, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.677-683.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Factor-Independent Assembly of Elongation-Competent Ribosomes by an Internal Ribosome Entry Site Located in an RNA Virus That Infects Penaeid Shrimp

Randal C. Cevallos and Peter Sarnow*

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California

Received 30 June 2004/ Accepted 30 August 2004

The Taura syndrome virus (TSV), a member of the Dicistroviridae family of viruses, is a single-stranded positive-sense RNA virus which contains two nonoverlapping reading frames separated by a 230-nucleotide intergenic region. This intergenic region contains an internal ribosome entry site (IRES) which directs the synthesis of the TSV capsid proteins. Unlike other dicistroviruses, the TSV IRES contains an AUG codon that is in frame with the capsid region, suggesting that the IRES initiates translation at this AUG codon by using initiator tRNAmet. We show here that the TSV IRES does not use this or any other AUG codon to initiate translation. Like the IRES in cricket paralysis virus (CrPV), the TSV IRES can assemble 80S ribosomes in the absence of initiation factors and can direct protein synthesis in a reconstituted system that contains only purified ribosomal subunits, eukaryotic elongation factors 1A and 2, and aminoacylated tRNAs. The functional conservation of the CrPV-like IRES elements in viruses that can infect different invertebrate hosts suggests that initiation at non-AUG codons by an initiation factor-independent mechanism may be more prevalent.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305. Phone: (650) 498-7076. Fax: (650) 498-7147. E-mail: psarnow{at}stanford.edu.

Journal of Virology, January 2005, p. 677-683, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.677-683.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pfingsten, J. S., Kieft, J. S. (2008). RNA structure-based ribosome recruitment: Lessons from the Dicistroviridae intergenic region IRESes. RNA 14: 1255-1263 [Abstract] [Full Text]  
  • Mokrejs, M., Vopalensky, V., Kolenaty, O., Masek, T., Feketova, Z., Sekyrova, P., Skaloudova, B., Kriz, V., Pospisek, M. (2006). IRESite: the database of experimentally verified IRES structures (www.iresite.org). Nucleic Acids Res 34: D125-D130 [Abstract] [Full Text]  
  • Komar, A. A., Hatzoglou, M. (2005). Internal Ribosome Entry Sites in Cellular mRNAs: Mystery of Their Existence. J. Biol. Chem. 280: 23425-23428 [Abstract] [Full Text]  


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