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Journal of Virology, September 2005, p. 12077-12080, Vol. 79, No. 18
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.18.12077-12080.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection

Drake C. Stenger,1* Roy French,1 and Frederick E. Gildow2

United States Department of Agriculture—Agricultural Research Service and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583,1 Department of Plant Pathology, Buckhout Laboratory, Pennsylvania State University, University Park, Pennsylvania 168022

Received 11 May 2005/ Accepted 28 June 2005

A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.

* Corresponding author. Mailing address: United States Department of Agriculture—Agricultural Research Service and Department of Plant Pathology, 344 Keim Hall, University of Nebraska, Lincoln, NE 68583. Phone: (402) 472-2710. Fax: (402) 472-4020. E-mail: dstenger{at}unlnotes.unl.edu.

Journal of Virology, September 2005, p. 12077-12080, Vol. 79, No. 18
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.18.12077-12080.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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