This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stenger, D. C.
Right arrow Articles by Gildow, F. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stenger, D. C.
Right arrow Articles by Gildow, F. E.

 Previous Article  |  Next Article 

Journal of Virology, September 2005, p. 12077-12080, Vol. 79, No. 18
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.18.12077-12080.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complete Deletion of Wheat Streak Mosaic Virus HC-Pro: a Null Mutant Is Viable for Systemic Infection

Drake C. Stenger,1* Roy French,1 and Frederick E. Gildow2

United States Department of Agriculture—Agricultural Research Service and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583,1 Department of Plant Pathology, Buckhout Laboratory, Pennsylvania State University, University Park, Pennsylvania 168022

Received 11 May 2005/ Accepted 28 June 2005

A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.

* Corresponding author. Mailing address: United States Department of Agriculture—Agricultural Research Service and Department of Plant Pathology, 344 Keim Hall, University of Nebraska, Lincoln, NE 68583. Phone: (402) 472-2710. Fax: (402) 472-4020. E-mail: dstenger{at}unlnotes.unl.edu.

Journal of Virology, September 2005, p. 12077-12080, Vol. 79, No. 18
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.18.12077-12080.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Torres-Barcelo, C., Martin, S., Daros, J.-A., Elena, S. F. (2008). From Hypo- to Hypersuppression: Effect of Amino Acid Substitutions on the RNA-Silencing Suppressor Activity of the Tobacco etch potyvirus HC-Pro. Genetics 180: 1039-1049 [Abstract] [Full Text]  
  • Cuellar, W. J., Tairo, F., Kreuze, J. F., Valkonen, J. P. T. (2008). Analysis of gene content in sweet potato chlorotic stunt virus RNA1 reveals the presence of the p22 RNA silencing suppressor in only a few isolates: implications for viral evolution and synergism. J. Gen. Virol. 89: 573-582 [Abstract] [Full Text]  
  • Valli, A., Dujovny, G., Garcia, J. A. (2008). Protease Activity, Self Interaction, and Small Interfering RNA Binding of the Silencing Suppressor P1b from Cucumber Vein Yellowing Ipomovirus. J. Virol. 82: 974-986 [Abstract] [Full Text]  
  • Shiboleth, Y. M., Haronsky, E., Leibman, D., Arazi, T., Wassenegger, M., Whitham, S. A., Gaba, V., Gal-On, A. (2007). The Conserved FRNK Box in HC-Pro, a Plant Viral Suppressor of Gene Silencing, Is Required for Small RNA Binding and Mediates Symptom Development. J. Virol. 81: 13135-13148 [Abstract] [Full Text]  
  • Valli, A., Lopez-Moya, J. J., Garcia, J. A. (2007). Recombination and gene duplication in the evolutionary diversification of P1 proteins in the family Potyviridae. J. Gen. Virol. 88: 1016-1028 [Abstract] [Full Text]  
  • Valli, A., Martin-Hernandez, A. M., Lopez-Moya, J. J., Garcia, J. A. (2006). RNA Silencing Suppression by a Second Copy of the P1 Serine Protease of Cucumber Vein Yellowing Ipomovirus, a Member of the Family Potyviridae That Lacks the Cysteine Protease HCPro.. J. Virol. 80: 10055-10063 [Abstract] [Full Text]  
  • Stenger, D. C., Young, B. A., French, R. (2006). Random mutagenesis of wheat streak mosaic virus HC-Pro: non-infectious interfering mutations in a gene dispensable for systemic infection of plants. J. Gen. Virol. 87: 2741-2747 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»