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Journal of Virology, September 2005, p. 11541-11546, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11541-11546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Importin 7 May Be Dispensable for Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus Infection of Primary Macrophages

Steven P. Zielske and Mario Stevenson*

Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Received 22 November 2004/ Accepted 3 June 2005

In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.

* Corresponding author. Mailing address: 373 Plantation St., Suite 319, University of Massachusetts Medical School, Worcester, MA 01605. Phone: (508) 856-3645. Fax: (508) 856-4075. E-mail: Mario.Stevenson{at}umassmed.edu.

Journal of Virology, September 2005, p. 11541-11546, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11541-11546.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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