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Journal of Virology, September 2005, p. 11517-11522, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11517-11522.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Quantitative Trait Loci for Susceptibility to Mouse Adenovirus Type 1

Amanda R. Welton,¹ Elissa J. Chesler,² Carla Sturkie,^3, Anne U. Jackson,⁴ Gwen N. Hirsch,^3, and Katherine R. Spindler^1*

Department of Microbiology and Immunology,¹ Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109,⁴ Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163,² Department of Genetics, University of Georgia, Athens, Georgia 30606³

Received 6 May 2005/ Accepted 10 June 2005

Adult SJL/J mice are highly susceptible to mouse adenovirus type 1 (MAV-1) infections, whereas other inbred strains, including BALB/cJ, are resistant (K. R. Spindler, L. Fang, M. L. Moore, C. C. Brown, G. N. Hirsch, and A. K. Kajon, J. Virol. 75:12039-12046, 2001). Using congenic mouse strains, we showed that the H-2^s haplotype of SJL/J mice is not associated with susceptibility to MAV-1. Susceptibility of MAV-1-infected (BALB/cJ x SJL/J)F₁ mice was intermediate between that of SJL/J mice and that of BALB/cJ mice, indicating that susceptibility is a genetically controlled quantitative trait. We mapped genetic loci involved in mouse susceptibility to MAV-1 by analysis of 192 backcross progeny in a genome scan with 65 simple sequence length polymorphic markers. A major quantitative trait locus (QTL) was detected on chromosome 15 (Chr 15) with a highly significant logarithm of odds score of 21. The locus on Chr 15 alone accounts for 40% of the total trait variance between susceptible and resistant strains. QTL modeling of the data indicated that there are a number of other QTLs with small effects that together with the major QTL on Chr 15 account for 54% of the trait variance. Identification of the major QTL is the first step in characterizing host genes involved in susceptibility to MAV-1.

Supplemental material for this article may be found at http://jvi.asm.org/.

Present address: Department of Horticulture, University of Georgia, Athens, Georgia.

Present address: Department of Animal and Dairy Science, University of Georgia, Athens, Georgia.