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Journal of Virology, September 2005, p. 11507-11512, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11507-11512.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Intracellular Localization of the Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein: Absence of Nucleolar Accumulation during Infection and after Expression as a Recombinant Protein in Vero Cells

Raymond R. R. Rowland,1* Vinita Chauhan,1 Ying Fang,2 Andrew Pekosz,3 Maureen Kerrigan,1 and Miriam D. Burton4

Diagnostic Medicine and Pathobiology, 1800 Denison Ave., Kansas State University, Manhattan, Kansas 66506,1 Department of Veterinary Science, South Dakota State University, Brookings, South Dakota 57007,2 Departments of Molecular Microbiology and Pathology & Immunology, Washington University School of Medicine, Campus Box 8230, 660 S. Euclid Ave., St. Louis, Missouri 63110,3 Department of Anatomy and Physiology, 1800 Denison Ave., Kansas State University, Manhattan, Kansas 665064

Received 7 April 2005/ Accepted 7 June 2005

The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.

* Corresponding author. Mailing address: Diagnostic Medicine and Pathobiology, 1800 Denison Ave., Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4631. Fax: (785) 532-4481. E-mail: browland{at}vet.ksu.edu.

Journal of Virology, September 2005, p. 11507-11512, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11507-11512.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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