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Journal of Virology, September 2005, p. 11382-11391, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11382-11391.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Adenovirus Type 5 E4orf3 Protein Targets the Mre11 Complex to Cytoplasmic Aggresomes

Felipe D. Araujo,1 Travis H. Stracker,1,2,# Christian T. Carson,1,2 Darwin V. Lee,1 and Matthew D. Weitzman1*

Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California 92037,1 Graduate Program, Department of Biology, University of California, San Diego, California 920932

Received 6 April 2005/ Accepted 1 June 2005

Virus infections have dramatic effects on structural and morphological characteristics of the host cell. The gene product of open reading frame 3 in the early region 4 (E4orf3) of adenovirus serotype 5 (Ad5) is involved in efficient replication and late protein synthesis. During infection with adenovirus mutants lacking the E4 region, the viral genomic DNA is joined into concatemers by cellular DNA repair factors, and this requires the Mre11/Rad50/Nbs1 complex. Concatemer formation can be prevented by the E4orf3 protein, which causes the cellular redistribution of the Mre11 complex. Here we show that E4orf3 colocalizes with components of the Mre11 complex in nuclear tracks and also in large cytoplasmic accumulations. Rearrangement of Mre11 and Rad50 by Ad5 E4orf3 is not dependent on interactions with Nbs1 or promyelocytic leukemia protein nuclear bodies. Late in infection the cytoplasmic inclusions appear as a distinct juxtanuclear accumulation at the centrosome and this requires an intact microtubule cytoskeleton. The large cytoplasmic accumulations meet the criteria defined for aggresomes, including {gamma}-tubulin colocalization and formation of a surrounding vimentin cage. E4orf3 also appears to alter the solubility of the cellular Mre11 complex. These data suggest that E4orf3 can target the Mre11 complex to an aggresome and may explain how the cellular repair complex is inactivated during adenovirus infection.

* Corresponding author. Mailing address: Laboratory of Genetics, Salk Institute for Biological Studies, P. O. Box 85800, San Diego, CA 92186-5800. Phone: (858) 453 4100, x2037. Fax: (858) 558 7454. E-mail: weitzman{at}salk.edu.

# Present address: Sloan Kettering Institute, 1275 York Ave, New York, NY 10021.

Journal of Virology, September 2005, p. 11382-11391, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11382-11391.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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