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Journal of Virology, August 2005, p. 10826-10829, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10826-10829.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bovine Viral Diarrhea Virus Entry Is Dependent on Clathrin-Mediated Endocytosis

Steve Lecot, Sandrine Belouzard, Jean Dubuisson, and Yves Rouillé*

CNRS-UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, Lille, France

Received 13 December 2004/ Accepted 21 May 2005

Cellular mechanisms of bovine viral diarrhea virus (BVDV) entry in MDBK cells were investigated. Chloroquine, bafilomycin A1, or ammonium chloride inhibited BVDV infection, indicating that an acidic endosomal pH is required for BVDV entry. The tyrosine kinase inhibitor genistein partially inhibited BVDV infection at a postentry step, whereas BVDV entry was strongly inhibited by chlorpromazine or by the overexpression of a dominant-negative form of EPS15, a protein essential for the formation of clathrin-coated vesicles at the plasma membrane. Together, these data indicate that BVDV infection requires an active clathrin-dependent endocytic pathway.

* Corresponding author. Mailing address: Unité Hépatite C, CNRS-UPR2511, Institut Pasteur de Lille, 1 rue Calmette, BP 447, 59021 Lille Cedex, France. Phone: (33) 3 20 87 10 27. Fax: (33) 3 20 87 12 01. E-mail: yves.rouille{at}ibl.fr.

Journal of Virology, August 2005, p. 10826-10829, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10826-10829.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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