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Journal of Virology, August 2005, p. 10498-10506, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10498-10506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Type 2 UL24 Gene Is a Virulence Determinant in Murine and Guinea Pig Disease Models

Susan Blakeney,1 Jacek Kowalski,1 Donna Tummolo,1 Joanne DeStefano,1 David Cooper,1 Min Guo,1 Seema Gangolli,1 Deborah Long,1 Timothy Zamb,1 Robert J. Natuk,1 and Robert J. Visalli1,2*

Wyeth Vaccines Research, Pearl River, New York 10965,1 Department of Biology, Indiana University-Purdue University Fort Wayne, Fort Wayne, Indiana 468052

Received 25 March 2005/ Accepted 4 May 2005

A herpes simplex virus type 2 (HSV-2) UL24 ß-glucuronidase (UL24-ßgluc) insertion mutant was derived from HSV-2 strain 186 via standard marker transfer techniques. Cell monolayers infected with UL24-ßgluc yielded cytopathic effect with syncytium formation. UL24-ßgluc replicated to wild-type viral titers in three different cell lines. UL24-ßgluc was not virulent after intravaginal inoculation of BALB/c mice in that all inoculated animals survived doses up to 400 times the 50% lethal dose (LD50) of the parental virus. Furthermore, few UL24-ßgluc-inoculated mice developed any vaginal lesions. Intravaginal inoculation of guinea pigs with UL24-ßgluc at a dose equivalent to the LD50 of parental virus ({approx}5 x 103 PFU) was not lethal (10/10 animals survived). Although genital lesions developed in some UL24-ßgluc-inoculated guinea pigs, both the overall number of lesions and the severity of disease were far less than that observed for animals infected with parental strain 186.

* Corresponding author. Present address: Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 E. Coliseum Blvd., Fort Wayne, IN 46805-1499. Phone: (260) 481-6320. Fax: (260) 481-6087. E-mail: visallir{at}ipfw.edu.

Journal of Virology, August 2005, p. 10498-10506, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10498-10506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bertrand, L., Pearson, A. (2008). The conserved N-terminal domain of herpes simplex virus 1 UL24 protein is sufficient to induce the spatial redistribution of nucleolin. J. Gen. Virol. 89: 1142-1151 [Abstract] [Full Text]  
  • Natuk, R. J., Cooper, D., Guo, M., Calderon, P., Wright, K. J., Nasar, F., Witko, S., Pawlyk, D., Lee, M., DeStefano, J., Tummolo, D., Abramovitz, A. S., Gangolli, S., Kalyan, N., Clarke, D. K., Hendry, R. M., Eldridge, J. H., Udem, S. A., Kowalski, J. (2006). Recombinant Vesicular Stomatitis Virus Vectors Expressing Herpes Simplex Virus Type 2 gD Elicit Robust CD4+ Th1 Immune Responses and Are Protective in Mouse and Guinea Pig Models of Vaginal Challenge. J. Virol. 80: 4447-4457 [Abstract] [Full Text]  


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