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Journal of Virology, August 2005, p. 10289-10299, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10289-10299.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Determinants of Infectious Pancreatic Necrosis Virus Virulence and Cell Culture Adaptation

Haichen Song,^1, Nina Santi,^2, Øystein Evensen,³ and Vikram N. Vakharia^1*

Center for Biosystems Research, University of Maryland Biotechnology Institute and VA-MD Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742,¹ Section for Pathology, National Veterinary Institute, N-0033 Oslo, Norway,² Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, N-0033 Oslo, Norway³

Received 18 January 2005/ Accepted 3 May 2005

Infectious pancreatic necrosis viruses (IPNVs) exhibit a wide range of virulence in salmonid species. In previous studies, we have shown that the amino acid residues at positions 217 and 221 in VP2 are implicated in virulence. To pinpoint the molecular determinants of virulence in IPNV, we generated recombinant IPNV strains using the cRNA-based reverse-genetics system. In two virulent strains, residues at positions 217 and 247 were replaced by the corresponding amino acids of a low-virulence strain. The growth characteristics of the recovered chimeric strains in cell culture were similar to the low-virulence strains, and these viruses induced significantly lower mortality in Atlantic salmon fry than the parent strains did in in vivo challenge studies. Furthermore, the virulent strain was serially passaged in CHSE-214 cells 10 times and was completely characterized by nucleotide sequencing. Deduced amino acid sequence analyses revealed a single amino acid substitution of Ala to Thr at position 221 in VP2 of this virus, which became highly attenuated and induced 15% cumulative mortality in Atlantic salmon fry, compared to 68% mortality induced by the virulent parent strain. The attenuated strain grows to higher titers in CHSE cells and can be distinguished antigenically from the wild-type virus by use of a monoclonal antibody. However, the virulent strain passaged 10 times in RTG-2 cells was stable, and it retained its antigenicity and virulence. Our results indicate that residues Thr at position 217 (Thr217) and Ala221 of VP2 are the major determinants of virulence in IPNV of the Sp serotype. Highly virulent isolates possess residues Thr217 and Ala221; moderate- to low-virulence strains have Pro217 and Ala221; and strains containing Thr221 are almost avirulent, irrespective of the residue at position 217.

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* Corresponding author. Mailing address: Center for Biosystems Research, 6126 Plant Science Building, University of Maryland, College Park, MD 20742. Phone: (301) 405-4777. Fax: (301) 314-9075. E-mail: vakharia{at}umbi.umd.edu.

H.S. and N.S. contributed equally to this work.

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Journal of Virology, August 2005, p. 10289-10299, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10289-10299.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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