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Journal of Virology, August 2005, p. 9685-9693, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9685-9693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Polerovirus Minor Capsid Protein Determines Vector Specificity and Intestinal Tropism in the Aphid

Véronique Brault,1* Sophie Périgon,1,{dagger} Catherine Reinbold,1 Monique Erdinger,1 Danièle Scheidecker,2 Etienne Herrbach,1 Ken Richards,2 and Véronique Ziegler-Graff2

Institut National de la Recherche Agronomique, 68021 Colmar, France,1 Institut de Biologie Moléculaire des Plantes, CNRS, 12 rue du Général Zimmer, 67084 Strasbourg, France2

Received 3 March 2005/ Accepted 9 April 2005

Aphid transmission of poleroviruses is highly specific, but the viral determinants governing this specificity are unknown. We used a gene exchange strategy between two poleroviruses with different vectors, Beet western yellows virus (BWYV) and Cucurbit aphid-borne yellows virus (CABYV), to analyze the role of the major and minor capsid proteins in vector specificity. Virus recombinants obtained by exchanging the sequence of the readthrough domain (RTD) between the two viruses replicated in plant protoplasts and in whole plants. The hybrid readthrough protein of chimeric viruses was incorporated into virions. Aphid transmission experiments using infected plants or purified virions revealed that vector specificity is driven by the nature of the RTD. BWYV and CABYV have specific intestinal sites in the vectors for endocytosis: the midgut for BWYV and both midgut and hindgut for CABYV. Localization of hybrid virions in aphids by transmission electron microscopy revealed that gut tropism is also determined by the viral origin of the RTD.

* Corresponding author. Mailing address: UMR INRA-Université L. Pasteur SVQV, BP 20507, 28 rue de Herrlisheim, 68021 Colmar Cedex, France. Phone: (33) 389224938. Fax: (33) 389224933. E-mail: brault{at}colmar.inra.fr.

{dagger} Present address: Ressources Naturelles Canada, Service Canadien des Forêts, Centre de Foresterie des Laurentides, 1055 rue du P.E.P.S., C.P. 3800 Sainte-Foy, Québec G1V 4C7, Canada.

Journal of Virology, August 2005, p. 9685-9693, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9685-9693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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