This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Österlund, P.
Right arrow Articles by Julkunen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Österlund, P.
Right arrow Articles by Julkunen, I.

 Previous Article  |  Next Article 

Journal of Virology, August 2005, p. 9608-9617, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9608-9617.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Gene Expression and Antiviral Activity of Alpha/Beta Interferons and Interleukin-29 in Virus-Infected Human Myeloid Dendritic Cells

Pamela Österlund,1 Ville Veckman,1 Jukka Sirén,1 Kevin M. Klucher,2 John Hiscott,3 Sampsa Matikainen,1 and Ilkka Julkunen1*

Department of Viral Diseases and Immunology, National Public Health Institute, FIN-00300 Helsinki, Finland,1 ZymoGenetics, Inc., Eastlake Ave. E., Seattle, Washington 98102,2 Lady Davis Institute for Medical Research and Departments of Microbiology and Immunology & Medicine, McGill University, Montreal, Quebec, Canada H3T 1E23

Received 5 January 2005/ Accepted 19 April 2005

Dendritic cells (DCs) respond to microbial infections by undergoing phenotypic maturation and by producing multiple cytokines. In the present study, we analyzed the ability of influenza A and Sendai viruses to induce DC maturation and activate tumor necrosis factor alpha (TNF-{alpha}), alpha/beta interferon (IFN-{alpha}/ß), and IFN-like interleukin-28A/B (IFN-{lambda}2/3) and IL-29 (IFN-{lambda}1) gene expression in human monocyte-derived myeloid DCs (mDC). The ability of influenza A virus to induce mDC maturation or enhance the expression of TNF-{alpha}, IFN-{alpha}/ß, interleukin-28 (IL-28), and IL-29 genes was limited, whereas Sendai virus efficiently induced mDC maturation and enhanced cytokine gene expression. Influenza A virus-induced expression of TNF-{alpha}, IFN-{alpha}, IFN-ß, IL-28, and IL-29 genes was, however, dramatically enhanced when cells were pretreated with IFN-{alpha}. IFN-{alpha} priming led to increased expression of Toll-like receptor 3 (TLR3), TLR7, TLR8, MyD88, TRIF, and IFN regulatory factor 7 (IRF7) genes and enhanced influenza-induced phosphorylation and DNA binding of IRF3. Influenza A virus also enhanced the binding of NF-{kappa}B to the respective NF-{kappa}B elements of the promoters of IFN-ß and IL-29 genes. In mDC IL-29 induced MxA protein expression and possessed antiviral activity against influenza A virus, although this activity was lower than that of IFN-{alpha} or IFN-ß. Our results show that in human mDCs viruses can readily induce the expression of IL-28 and IL-29 genes whose gene products are likely to contribute to the host antiviral response.

* Corresponding author. Mailing address: Department of Viral Diseases and Immunology, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358-9-47448372. Fax: 358-9-47448355. E-mail: ilkka.julkunen{at}ktl.fi.

Journal of Virology, August 2005, p. 9608-9617, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9608-9617.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, M., Liu, X., Zhou, Y., Su, S. B. (2009). Interferon-{lambda}s: the modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol. 86: 23-32 [Abstract] [Full Text]  
  • Ohman, T., Rintahaka, J., Kalkkinen, N., Matikainen, S., Nyman, T. A. (2009). Actin and RIG-I/MAVS Signaling Components Translocate to Mitochondria upon Influenza A Virus Infection of Human Primary Macrophages. J. Immunol. 182: 5682-5692 [Abstract] [Full Text]  
  • Mikkelsen, S. S., Jensen, S. B., Chiliveru, S., Melchjorsen, J., Julkunen, I., Gaestel, M., Arthur, J. S. C., Flavell, R. A., Ghosh, S., Paludan, S. R. (2009). RIG-I-mediated Activation of p38 MAPK Is Essential for Viral Induction of Interferon and Activation of Dendritic Cells: DEPENDENCE ON TRAF2 AND TAK1. J. Biol. Chem. 284: 10774-10782 [Abstract] [Full Text]  
  • Hou, W., Wang, X., Ye, L., Zhou, L., Yang, Z.-Q., Riedel, E., Ho, W.-Z. (2009). Lambda Interferon Inhibits Human Immunodeficiency Virus Type 1 Infection of Macrophages. J. Virol. 83: 3834-3842 [Abstract] [Full Text]  
  • Makela, S. M., Strengell, M., Pietila, T. E., Osterlund, P., Julkunen, I. (2009). Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte-derived macrophages and dendritic cells. J. Leukoc. Biol. 85: 664-672 [Abstract] [Full Text]  
  • Larange, A., Antonios, D., Pallardy, M., Kerdine-Romer, S. (2009). TLR7 and TLR8 agonists trigger different signaling pathways for human dendritic cell maturation. J. Leukoc. Biol. 85: 673-683 [Abstract] [Full Text]  
  • Wang, J., Oberley-Deegan, R., Wang, S., Nikrad, M., Funk, C. J., Hartshorn, K. L., Mason, R. J. (2009). Differentiated Human Alveolar Type II Cells Secrete Antiviral IL-29 (IFN-{lambda}1) in Response to Influenza A Infection. J. Immunol. 182: 1296-1304 [Abstract] [Full Text]  
  • Sillanpaa, M., Kaukinen, P., Melen, K., Julkunen, I. (2008). Hepatitis C virus proteins interfere with the activation of chemokine gene promoters and downregulate chemokine gene expression. J. Gen. Virol. 89: 432-443 [Abstract] [Full Text]  
  • Veckman, V., Julkunen, I. (2008). Streptococcus pyogenes activates human plasmacytoid and myeloid dendritic cells. J. Leukoc. Biol. 83: 296-304 [Abstract] [Full Text]  
  • Osterlund, P. I., Pietila, T. E., Veckman, V., Kotenko, S. V., Julkunen, I. (2007). IFN Regulatory Factor Family Members Differentially Regulate the Expression of Type III IFN (IFN-{lambda}) Genes. J. Immunol. 179: 3434-3442 [Abstract] [Full Text]  
  • Onoguchi, K., Yoneyama, M., Takemura, A., Akira, S., Taniguchi, T., Namiki, H., Fujita, T. (2007). Viral Infections Activate Types I and III Interferon Genes through a Common Mechanism. J. Biol. Chem. 282: 7576-7581 [Abstract] [Full Text]  
  • Remoli, M. E., Ragimbeau, J., Giacomini, E., Gafa, V., Severa, M., Lande, R., Pellegrini, S., Coccia, E. M. (2007). NF-{kappa}B is required for STAT-4 expression during dendritic cell maturation. J. Leukoc. Biol. 81: 355-363 [Abstract] [Full Text]  
  • Pietila, T. E., Veckman, V., Lehtonen, A., Lin, R., Hiscott, J., Julkunen, I. (2007). Multiple NF-{kappa}B and IFN Regulatory Factor Family Transcription Factors Regulate CCL19 Gene Expression in Human Monocyte-Derived Dendritic Cells. J. Immunol. 178: 253-261 [Abstract] [Full Text]  
  • Kornbluth, R. S., Stone, G. W. (2006). Immunostimulatory combinations: designing the next generation of vaccine adjuvants. J. Leukoc. Biol. 80: 1084-1102 [Abstract] [Full Text]  
  • Fernandez-Sesma, A., Marukian, S., Ebersole, B. J., Kaminski, D., Park, M.-S., Yuen, T., Sealfon, S. C., Garcia-Sastre, A., Moran, T. M. (2006). Influenza Virus Evades Innate and Adaptive Immunity via the NS1 Protein.. J. Virol. 80: 6295-6304 [Abstract] [Full Text]  
  • Ank, N., West, H., Bartholdy, C., Eriksson, K., Thomsen, A. R., Paludan, S. R. (2006). Lambda Interferon (IFN-{lambda}), a Type III IFN, Is Induced by Viruses and IFNs and Displays Potent Antiviral Activity against Select Virus Infections In Vivo. J. Virol. 80: 4501-4509 [Abstract] [Full Text]  
  • Ziegler, T., Matikainen, S., Ronkko, E., Osterlund, P., Sillanpaa, M., Siren, J., Fagerlund, R., Immonen, M., Melen, K., Julkunen, I. (2005). Severe Acute Respiratory Syndrome Coronavirus Fails To Activate Cytokine-Mediated Innate Immune Responses in Cultured Human Monocyte-Derived Dendritic Cells. J. Virol. 79: 13800-13805 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»