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Journal of Virology, August 2005, p. 10077-10082, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.10077-10082.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Immediate-Early Protein IE0 of the Autographa californica Nucleopolyhedrovirus Is Not Essential for Viral Replication{dagger}

Lu Liqun,{ddagger} Hadassah Rivkin, and Nor Chejanovsky*

Entomology Department, Institute of Plant Protection, ARO, The Volcani Center, POB 6 Bet Dagan 50250, Israel

Received 2 July 2004/ Accepted 25 April 2005

The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAc{Delta}ie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAc{Delta}ie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAc{Delta}ie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644, 2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells.

* Corresponding author. Mailing address: Entomology Department, The Institute of Plant Protection, The Volcani Center, POB 6, Bet Dagan 50250, Israel. Phone: 972-3-968-3694. Fax: 972-3-960-4180. E-mail: ninajiq{at}volcani.agri.gov.il.

{dagger} Contribution 507/04 from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.

{ddagger} Present address: Animal Health Biotechnology Unit, Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.

Journal of Virology, August 2005, p. 10077-10082, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.10077-10082.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schultz, K. L. W., Wetter, J. A., Fiore, D. C., Friesen, P. D. (2009). Transactivator IE1 Is Required for Baculovirus Early Replication Events That Trigger Apoptosis in Permissive and Nonpermissive Cells. J. Virol. 83: 262-272 [Abstract] [Full Text]  
  • Fang, M., Dai, X., Theilmann, D. A. (2007). Autographa californica Multiple Nucleopolyhedrovirus EXON0 (ORF141) Is Required for Efficient Egress of Nucleocapsids from the Nucleus. J. Virol. 81: 9859-9869 [Abstract] [Full Text]  
  • Rivkin, H., Kroemer, J. A., Bronshtein, A., Belausov, E., Webb, B. A., Chejanovsky, N. (2006). Response of immunocompetent and immunosuppressed Spodoptera littoralis larvae to baculovirus infection.. J. Gen. Virol. 87: 2217-2225 [Abstract] [Full Text]  


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