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Journal of Virology, July 2005, p. 9341-9345, Vol. 79, No. 14
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.14.9341-9345.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

RNA-Dependent Protein Kinase Is Required for Alpha-1 Interferon Transgene-Induced Resistance to Genital Herpes Simplex Virus Type 2

Daniel J. J. Carr,1* Lisa Tomanek,1 Robert H. Silverman,2 Iain L. Campbell,3 and Bryan R. G. Williams2

Department of Ophthalmology, Microbiology, and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104,1 Department of Cancer Biology/NB40, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195,2 School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia3

Received 22 February 2005/ Accepted 28 March 2005

We investigated the mechanism of resistance to genital herpes simplex virus type 2 (HSV-2) infection in mice transfected with the murine alpha-1 interferon (IFN-{alpha}1) transgene. In situ transfection of mice with the IFN-{alpha}1 transgene resulted in an elevation in an IFN-responsive gene, RNA-dependent protein kinase (PKR), but not 2',5'-oligoadenylate synthetases (OAS), in vaginal tissue. Coupled with the finding that mice lacking a functional PKR pathway were no longer resistant to genital HSV-2 infection following transfection with the IFN-{alpha}1 transgene in comparison to wild-type mice or mice lacking a functional OAS pathway, these results suggest that PKR is the dominant antiviral pathway activated by the IFN-{alpha}1 transgene.

* Corresponding author. Mailing address: Department of Ophthalmology, DMEI #415, The University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., Oklahoma City, OK 73104. Phone: (405) 271-8784. Fax: (405) 271-8781. E-mail: dan-carr{at}ouhsc.edu.

Journal of Virology, July 2005, p. 9341-9345, Vol. 79, No. 14
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.14.9341-9345.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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