This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ahn, J.
Right arrow Articles by Lee, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahn, J.
Right arrow Articles by Lee, H.

 Previous Article  |  Next Article 

Journal of Virology, July 2005, p. 8620-8624, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8620-8624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Small Interfering RNA Targeting Coxsackievirus B3 Protects Permissive HeLa Cells from Viral Challenge

Jeonghyun Ahn,1 Eun Seok Jun,2,3 Hui Sun Lee,1 Seung Yong Yoon,4 DongHou Kim,4,5 Chul-Hyun Joo,1,5 Yoo Kyum Kim,1,5 and Heuiran Lee1,5*

Departments of Microbiology,1 Anatomy & Cell Biology,4 Research Institute for Biomacromolecules, University of Ulsan College of Medicine,5 Department of Medicine, Sungkyunkwan University School of Medicine,2 Cardiac and Vascular Center, Samsung Medical Center, Seoul, Korea3

Received 10 December 2004/ Accepted 1 March 2005

We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal antienterovirus strategy.

* Corresponding author. Mailing address: University of Ulsan College of Medicine, Department of Microbiology, University of Ulsan College of Medicine, Songpa, P.O. Box 145, Seoul, Korea. Phone: 82-2-3010-4286. Fax: 82-2-485-2320. E-mail: heuiran{at}amc.seoul.kr.

Journal of Virology, July 2005, p. 8620-8624, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8620-8624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stone, J. K., Rijnbrand, R., Stein, D. A., Ma, Y., Yang, Y., Iversen, P. L., Andino, R. (2008). A Morpholino Oligomer Targeting Highly Conserved Internal Ribosome Entry Site Sequence Is Able To Inhibit Multiple Species of Picornavirus. Antimicrob. Agents Chemother. 52: 1970-1981 [Abstract] [Full Text]  
  • Lee, H. S., Ahn, J., Jee, Y., Seo, I. S., Jeon, E. J., Jeon, E.-S., Joo, C. H., Kim, Y. K., Lee, H. (2007). Universal and mutation-resistant anti-enteroviral activity: potency of small interfering RNA complementary to the conserved cis-acting replication element within the enterovirus coding region. J. Gen. Virol. 88: 2003-2012 [Abstract] [Full Text]  
  • Kusov, Y., Kanda, T., Palmenberg, A., Sgro, J.-Y., Gauss-Muller, V. (2006). Silencing of Hepatitis A Virus Infection by Small Interfering RNAs.. J. Virol. 80: 5599-5610 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»