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Journal of Virology, July 2005, p. 8470-8479, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8470-8479.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus 1 ICP22 Regulates the Accumulation of a Shorter mRNA and of a Truncated U_S3 Protein Kinase That Exhibits Altered Functions

The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637

Received 19 January 2005/ Accepted 22 February 2005

The U_S3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U_S3 gene encodes two proteins. The predominant U_S3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein designated U_S3.5 and initiating from methionine 77 were preeminent in cells infected with a mutant lacking the gene encoding ICP22. Both the wild-type and truncated proteins also accumulated in cells transduced with a baculovirus carrying the entire U_S3 open reading frame. The U_S3.5 protein accumulating in cells infected with the mutant lacking the gene encoding ICP22 mediated the phosphorylation of histone deacetylase 1, a function of U_S3 protein, but failed to block apoptosis of the infected cells. The U_S3.5 and U_S3 proteins differ with respect to the range of functions they exhibit.

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