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Journal of Virology, July 2005, p. 8230-8236, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8230-8236.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

New DNA Viruses Identified in Patients with Acute Viral Infection Syndrome

Morris S. Jones,1,2 Amit Kapoor,1,2 Vladimir V. Lukashov,3,4 Peter Simmonds,5 Frederick Hecht,2,6 and Eric Delwart1,2*

Blood Systems Research Institute, San Francisco, California 94118,1 Department of Medicine, University of California, San Francisco, California 94118,2 Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, 1105AZ Amsterdam, The Netherlands,3 D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 123098 Moscow, Russia,4 Centre for Infectious Diseases, University of Edinburgh, Summerhall EH9 1AJ, United Kingdom,5 Positive Health Program HIV, San Francisco General Hospital, San Francisco, California6

Received 12 July 2004/ Accepted 29 November 2004

A sequence-independent PCR amplification method was used to identify viral nucleic acids in the plasma samples of 25 individuals presenting with symptoms of acute viral infection following high-risk behavior for human immunodeficiency virus type 1 transmission. GB virus C/hepatitis G virus was identified in three individuals and hepatitis B virus in one individual. Three previously undescribed DNA viruses were also detected, a parvovirus and two viruses related to TT virus (TTV). Nucleic acids in human plasma that were distantly related to bacterial sequences or with no detectable similarities to known sequences were also found. Nearly complete viral genome sequencing and phylogenetic analysis confirmed the presence of a new parvovirus distinct from known human and animal parvoviruses and of two related TTV-like viruses highly divergent from both the TTV and TTV-like minivirus groups. The detection of two previously undescribed viral species in a small group of individuals presenting acute viral syndrome with unknown etiology indicates that a rich yield of new human viruses may be readily identifiable using simple methods of sequence-independent nucleic acid amplification and limited sequencing.

* Corresponding author. Mailing address: Blood Systems Research Institute, 270 Masonic Ave., San Francisco, CA 94118. Phone: (415) 923-5763. Fax: (419) 791-4220. E-mail: delwarte{at}medicine.ucsf.edu.

Journal of Virology, July 2005, p. 8230-8236, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8230-8236.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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