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Journal of Virology, June 2005, p. 7911-7917, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7911-7917.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Nonstructural Protein Membrane Anchor Deletion Mutants Expressed in the Context of the Hepatitis C Virus Polyprotein

Rainer Gosert,1,2,{dagger} Wiebke Jendrsczok,1,{dagger} Jan Martin Berke,1,3 Volker Brass,1 Hubert E. Blum,1 and Darius Moradpour1,3*

Department of Medicine II, University of Freiburg, Hugstetter Strasse 55, D-79106 Freiburg, Germany,1 Institute for Medical Microbiology, University of Basel, Petersplatz 10, CH-4003 Basel,2 Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011 Lausanne, Switzerland3

Received 20 July 2004/ Accepted 18 February 2005

Protein-protein interactions involved in formation of the membrane-associated hepatitis C virus (HCV) replication complex are poorly understood. Here, we investigated nonstructural proteins with deletions in their membrane anchor domains when expressed in the context of the entire HCV polyprotein. Interactions among cytosolic domains of HCV nonstructural proteins were found not to be sufficiently strong to rescue such mutants to the membrane. Thus, the membrane anchor domains of nonstructural proteins are essential for incorporation of these proteins into the HCV replication complex while interactions among the cytosolic domains appear to be relatively weak. This feature may provide the nonstructural proteins with a certain flexibility to perform their multiple functions during HCV replication.

* Corresponding author. Mailing address: Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland. Phone: 41 21 314 47 23. Fax: 41 21 314 47 18. E-mail: Darius.Moradpour{at}hospvd.ch.

{dagger} R.G. and W.J. contributed equally to this work.

Journal of Virology, June 2005, p. 7911-7917, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7911-7917.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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