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Journal of Virology, June 2005, p. 7877-7882, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7877-7882.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of STAT 1 Phosphorylation by Human Parainfluenza Virus Type 3 C Protein

Achut G. Malur,1 Santanu Chattopadhyay,1 Ratan K. Maitra,2 and Amiya K. Banerjee1*

Department of Molecular Biology, Section Virology NN-10,1 Virus Core Facility, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 441952

Received 8 December 2004/ Accepted 23 February 2005

The P mRNA of the viruses belonging to the subfamily Paramyxovirinae possesses a unique property of giving rise to several accessory proteins by a process that involves the utilization of overlapping open reading frames (the C proteins) and by an "RNA-editing" mechanism (the V proteins). Although these proteins are considered accessory, numerous studies have highlighted the importance of these proteins in virus transcription and interferon signaling, including our previous observation on the role of human parainfluenza virus type 3 (HPIV 3) C protein in the transcription of viral genome (Malur et al., Virus Res. 99:199-204, 2004). In this report, we have addressed its role in interferon signaling by generating a stable cell line, L-C6, by using the lentiviral expression system which expresses HPIV 3 C protein. The L-C6 cells were efficient in abrogating both alpha and gamma interferon-induced antiviral states and demonstrated a drastic reduction in the formation of gamma-activated factor complexes in the cell extracts. Western blot analysis subsequently revealed a defect in the phosphorylation of STAT 1 in these cells. Taken together, our results indicate that HPIV 3 C protein is capable of counteracting the interferon signaling pathway by specifically inhibiting the activation of STAT 1.

* Corresponding author. Mailing address: Cleveland Clinic Foundation, Section Virology NN-10, Department of Molecular Biology, 9500 Euclid Avenue, Cleveland, OH 44195-5178. Phone: (216) 444-0625. Fax: (216) 444-2998. E-mail: banerja{at}ccf.org.

Journal of Virology, June 2005, p. 7877-7882, Vol. 79, No. 12
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.12.7877-7882.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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