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Journal of Virology, June 2005, p. 7273-7278, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7273-7278.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein

Oliver Schildgen, Sascha Gräper,{dagger} Johannes Blümel,{ddagger} and Bertfried Matz*

Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Bonn, Germany

Received 30 November 2004/ Accepted 7 January 2005

Genome replication of herpes simplex viruses (HSV) in cultured cells is thought to be started by the action of the virus-encoded origin-binding protein (OBP). In experiments using two HSV-1 mutants with temperature-sensitive lesions in the helicase domain of OBP, we demonstrated that this function is essential during the first 6 hours of the lytic cycle. Once DNA synthesis has started, this function is no longer required, suggesting that origin-driven initiation of viral DNA replication is a single event rather than a continuous process.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany. Phone: (49)-228-287-5881. Fax: (49)-228-287-4433. E-mail: bertfried.matz{at}ukb.uni-bonn.de.

{dagger} Present address: Aventis Pharma, Industriepark Hoechst, Gebäude G650, D-65926 Frankfurt a.M., Germany.

{ddagger} Present address: Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.

Journal of Virology, June 2005, p. 7273-7278, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7273-7278.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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