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Journal of Virology, June 2005, p. 7255-7261, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7255-7261.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Heat Shock Protein 90 Expression in Epstein-Barr Virus-Infected B Cells Promotes T-Cell Proliferation In Vitro

Maria Kotsiopriftis, Jerome E. Tanner, and Caroline Alfieri^*

Sainte-Justine Hospital Research Center and Department of Microbiology and Immunology, Université de Montréal, Montréal, Québec H3T 1C5, Canada

Received 12 July 2004/ Accepted 20 January 2005

The aim of this study was to elucidate the in vitro response of T cells to Epstein-Barr virus (EBV)-infected B cells and to determine whether EBV-induced heat shock proteins (HSPs) might serve as T-cell stimulants. Cytofluorometric analysis revealed HSP90 cell surface expression in 12% of the EBV-immortalized B-cell population in all four of the B-cell lines tested. HSP27, HSP60, and HSP70 were not detected on the cell surface by cytofluorometry in these same B-cell lines. HSP90 and HSP60, but not HSP70 or HSP27, were detected on the cell surface after ¹²⁵I cell surface labeling and immunoprecipitation with anti-human HSP monoclonal antibodies. In vitro kinetic studies indicated that T cells increased at least twofold by day 11 postinfection in cultures of EBV-seronegative peripheral blood lymphocytes infected with EBV, whereas percentages of ß T cells in these same cultures either decreased slightly or remained relatively unchanged in response to EBV infection. Addition of anti-human HSP90 monoclonal antibody to the EBV-infected lymphocyte cultures inhibited T-cell expansion by 92%. The inhibition of T-cell expansion by anti-HSP90 antibody was reversed upon treatment with exogenous HSP90. Taken together, these results indicate that HSP90 played an important role in the stimulation of T cells during EBV infection of B cells in vitro and may serve as an important immunomodulator of T cells during acute EBV infection.

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* Corresponding author. Mailing address: Sainte-Justine Hospital Research Center, 3175 Côte Sainte-Catherine Road, Montréal (Québec), Canada H3T 1C5. Phone: (514) 345-4931, ext. 6135. Fax: (514) 345-4801. E-mail: carolina.alfieri{at}recherche-ste-justine.qc.ca.

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Journal of Virology, June 2005, p. 7255-7261, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7255-7261.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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