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Journal of Virology, June 2005, p. 7172-7181, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7172-7181.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutational Analysis of Bovine Leukemia Virus Rex: Identification of a Dominant-Negative Inhibitor

Eun-A Choi and Thomas J. Hope*

Department of Microbiology and Immunology, University of Illinois at Chicago, 835 S. Wolcott, Chicago, Illinois 60612

Received 21 June 2004/ Accepted 21 January 2005

The Rex proteins of the delta-retroviruses act to facilitate the export of intron-containing viral RNAs. The Rex of bovine leukemia virus (BLV) is poorly characterized. To gain a better understanding of BLV Rex, we generated a reporter assay to measure BLV Rex function and used it to screen a series of point and deletion mutations. Using this approach, we were able to identify the nuclear export signal of BLV Rex. Further, we identified a dominant-negative form of BLV Rex. Protein localization analysis revealed that wild-type BLV Rex had a punctate nuclear localization and was associated with nuclear pores. In contrast, the dominant-negative BLV Rex mutation had a diffuse nuclear localization and no nuclear pore association. Overexpression of the dominant-negative BLV Rex altered the localization of the wild-type protein. This dominant-negative derivative of BLV Rex could be a useful tool to test the concept of intracellular immunization against viral infection in a large animal model.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Illinois at Chicago, 835 S. Wolcott, Chicago, IL 60612. Phone: (312) 413-3424. Fax: (312) 996-6415. E-mail: thope{at}uic.edu.

Journal of Virology, June 2005, p. 7172-7181, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.7172-7181.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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