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Journal of Virology, June 2005, p. 6808-6813, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.6808-6813.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Protein Kinase C {theta} Is Not Essential for T-Cell-Mediated Clearance of Murine Gammaherpesvirus 68

Francesca Giannoni,{dagger} Ashley B. Lyon,{dagger} Mark D. Wareing,{ddagger} Peter B. Dias,{dagger} and Sally R. Sarawar*

Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121

Received 2 November 2004/ Accepted 18 January 2005

Murine gammaherpesvirus 68 (MHV-68) is a naturally occurring rodent pathogen with significant homology to human pathogens Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. T cells are essential for primary clearance of MHV-68 and survival of mice following intranasal infection. Previous reports have suggested that protein kinase C {theta} (PKC{theta}) is essential for T-cell activation and cytokine production in vitro. To determine the role of this molecule in vivo during the immune response to a viral infection, PKC{theta}–/– mice were infected with MHV-68. Despite the essential role of T cells in viral clearance, PKC{theta}–/– mice survived infection, cleared lytic virus, and maintained effective long-term control of latency. CD8 T-cell expansion, trafficking to the lung, and cytotoxic activity were similar in PKC{theta}+/+ and PKC{theta}–/– mice, whereas antiviral antibody and T-helper cell cytokine production were significantly lower in PKC{theta}–/– mice than in PKC{theta}+/+ mice. These studies demonstrate a differential requirement for PKC{theta} in the immune response to MHV-68 and show that PKC{theta} is not essential for the T-cell activation events leading to viral clearance.

* Corresponding author. Present address: Viral Immunology, Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121. Phone: (858) 909-5139. Fax: (858) 909-5141. E-mail: ssarawar{at}tpims.org.

{dagger} Present address: Viral Immunology, Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121.

{ddagger} Present address: Department of Medicine, Karolinska Institute, Huddinge University Hospital, SE-141 86 Stockholm, Sweden.

Journal of Virology, June 2005, p. 6808-6813, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.6808-6813.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dias, P., Shea, A. L., Inglis, C., Giannoni, F., Lee, L. N., Sarawar, S. R. (2008). Primary Clearance of Murine Gammaherpesvirus 68 by PKC{theta}-/- CD8 T Cells Is Compromised in the Absence of Help from CD4 T Cells. J. Virol. 82: 11970-11975 [Abstract] [Full Text]  
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