This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, P. Articles by Jiang, X. Search for Related Content PubMed PubMed Citation Articles by Huang, P. Articles by Jiang, X.

 Previous Article  |  Next Article 

Journal of Virology, June 2005, p. 6714-6722, Vol. 79, No. 11
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.11.6714-6722.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Norovirus and Histo-Blood Group Antigens: Demonstration of a Wide Spectrum of Strain Specificities and Classification of Two Major Binding Groups among Multiple Binding Patterns

Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center,¹ University of Cincinnati College of Medicine, Cincinnati, Ohio,² Navy Environmental and Preventive Medicine Unit 6, Pearl Harbor, Hawaii³

Received 24 September 2004/ Accepted 15 January 2005

Noroviruses, an important cause of acute gastroenteritis, have been found to recognize human histo-blood group antigens (HBGAs) as receptors. Four strain-specific binding patterns to HBGAs have been described in our previous report. In this study, we have extended the binding patterns to seven based on 14 noroviruses examined. The oligosaccharide-based assays revealed additional epitopes that were not detected by the saliva-based assays. The seven patterns have been classified into two groups according to their interactions with three major epitopes (A/B, H, and Lewis) of human HBGAs: the A/B-binding group and the Lewis-binding group. Strains in the A/B binding group recognize the A and/or B and H antigens, but not the Lewis antigens, while strains in the Lewis-binding group react only to the Lewis and/or H antigens. This classification also resulted in a model of the norovirus/HBGA interaction. Phylogenetic analyses showed that strains with identical or closely related binding patterns tend to be clustered, but strains in both binding group can be found in both genogroups I and II. Our results suggest that noroviruses have a wide spectrum of host range and that human HBGAs play an important role in norovirus evolution. The high polymorphism of the human HBGA system, the involvement of multiple epitopes, and the typical protein/carbohydrate interaction between norovirus VLPs and HBGAs provide an explanation for the virus-ligand binding diversities.

This article has been cited by other articles:

• Tian, P., Engelbrektson, A., Mandrell, R. (2008). Two-Log Increase in Sensitivity for Detection of Norovirus in Complex Samples by Concentration with Porcine Gastric Mucin Conjugated to Magnetic Beads. Appl. Environ. Microbiol. 74: 4271-4276 [Abstract] [Full Text]  
• Choi, J.-M., Hutson, A. M., Estes, M. K., Prasad, B. V. V. (2008). Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus. Proc. Natl. Acad. Sci. USA 105: 9175-9180 [Abstract] [Full Text]  
• Siebenga, J. J., Vennema, H., Renckens, B., de Bruin, E., van der Veer, B., Siezen, R. J., Koopmans, M. (2007). Epochal Evolution of GGII.4 Norovirus Capsid Proteins from 1995 to 2006. J. Virol. 81: 9932-9941 [Abstract] [Full Text]  
• Cao, S., Lou, Z., Tan, M., Chen, Y., Liu, Y., Zhang, Z., Zhang, X. C., Jiang, X., Li, X., Rao, Z. (2007). Structural Basis for the Recognition of Blood Group Trisaccharides by Norovirus. J. Virol. 81: 5949-5957 [Abstract] [Full Text]  
• Cheetham, S., Souza, M., McGregor, R., Meulia, T., Wang, Q., Saif, L. J. (2007). Binding Patterns of Human Norovirus-Like Particles to Buccal and Intestinal Tissues of Gnotobiotic Pigs in Relation to A/H Histo-Blood Group Antigen Expression. J. Virol. 81: 3535-3544 [Abstract] [Full Text]  
• Feng, X., Jiang, X. (2007). Library Screen for Inhibitors Targeting Norovirus Binding to Histo-Blood Group Antigen Receptors. Antimicrob. Agents Chemother. 51: 324-331 [Abstract] [Full Text]  
• Tan, M., Meller, J., Jiang, X. (2006). C-terminal arginine cluster is essential for receptor binding of norovirus capsid protein.. J. Virol. 80: 7322-7331 [Abstract] [Full Text]  
• Wobus, C. E., Thackray, L. B., Virgin, H. W. IV (2006). Murine norovirus: a model system to study norovirus biology and pathogenesis.. J. Virol. 80: 5104-5112 [Full Text]  
• Tan, M., Jiang, X. (2005). The P Domain of Norovirus Capsid Protein Forms a Subviral Particle That Binds to Histo-Blood Group Antigen Receptors. J. Virol. 79: 14017-14030 [Abstract] [Full Text]