This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Santra, S. Articles by Letvin, N. L. Search for Related Content PubMed PubMed Citation Articles by Santra, S. Articles by Letvin, N. L.

Replication-Defective Adenovirus Serotype 5 Vectors Elicit Durable Cellular and Humoral Immune Responses in Nonhuman Primates

Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115,¹ Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-3005²

Received 22 November 2004/ Accepted 5 January 2005

The magnitude and durability of immune responses induced by replication-defective adenovirus serotype 5 (ADV5) vector-based vaccines were evaluated in the simian-human immunodeficiency virus/rhesus monkey model. A single inoculation of recombinant ADV5 vector constructs induced cellular and humoral immunity, but the rapid generation of neutralizing anti-Ad5 antibodies limited the immunity induced by repeated vector administration. The magnitude and durability of the immune responses elicited by these vaccines were greater when they were delivered as boosting immunogens in plasmid DNA-primed monkeys than when they were used as single-modality immunogens. Therefore, administration of ADV5-based vectors in DNA-primed subjects may be a preferred use of this vaccine modality for generating long-term immune protection.

This article has been cited by other articles:

• Liu, J., Ewald, B. A., Lynch, D. M., Denholtz, M., Abbink, P., Lemckert, A. A. C., Carville, A., Mansfield, K. G., Havenga, M. J., Goudsmit, J., Barouch, D. H. (2008). Magnitude and Phenotype of Cellular Immune Responses Elicited by Recombinant Adenovirus Vectors and Heterologous Prime-Boost Regimens in Rhesus Monkeys. J. Virol. 82: 4844-4852 [Abstract] [Full Text]  
• Sridhar, S., Reyes-Sandoval, A., Draper, S. J., Moore, A. C., Gilbert, S. C., Gao, G. P., Wilson, J. M., Hill, A. V. S. (2008). Single-Dose Protection against Plasmodium berghei by a Simian Adenovirus Vector Using a Human Cytomegalovirus Promoter Containing Intron A. J. Virol. 82: 3822-3833 [Abstract] [Full Text]  
• Rodriguez, A., Goudsmit, J., Companjen, A., Mintardjo, R., Gillissen, G., Tax, D., Sijtsma, J., Weverling, G. J., Holterman, L., Lanar, D. E., Havenga, M. J. E., Radosevic, K. (2008). Impact of Recombinant Adenovirus Serotype 35 Priming versus Boosting of a Plasmodium falciparum Protein: Characterization of T- and B-Cell Responses to Liver-Stage Antigen 1. Infect. Immun. 76: 1709-1718 [Abstract] [Full Text]  
• Worgall, S., Krause, A., Qiu, J., Joh, J., Hackett, N. R., Crystal, R. G. (2007). Protective Immunity to Pseudomonas aeruginosa Induced with a Capsid-Modified Adenovirus Expressing P. aeruginosa OprF. J. Virol. 81: 13801-13808 [Abstract] [Full Text]  
• Barker, B. R., Parvani, J. G., Meyer, D., Hey, A. S., Skak, K., Letvin, N. L. (2007). IL-21 Induces Apoptosis of Antigen-Specific CD8+ T Lymphocytes. J. Immunol. 179: 3596-3603 [Abstract] [Full Text]  
• Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy, K. C., Hensley, S. E., Zhou, D., Lin, S.-W., Bian, A., Xiang, Z. Q., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., Ertl, H. C. J. (2007). Adenoviral vectors persist in vivo and maintain activated CD8+ T cells: implications for their use as vaccines. Blood 110: 1916-1923 [Abstract] [Full Text]  
• Radosevic, K., Wieland, C. W., Rodriguez, A., Weverling, G. J., Mintardjo, R., Gillissen, G., Vogels, R., Skeiky, Y. A. W., Hone, D. M., Sadoff, J. C., van der Poll, T., Havenga, M., Goudsmit, J. (2007). Protective Immune Responses to a Recombinant Adenovirus Type 35 Tuberculosis Vaccine in Two Mouse Strains: CD4 and CD8 T-Cell Epitope Mapping and Role of Gamma Interferon. Infect. Immun. 75: 4105-4115 [Abstract] [Full Text]  
• Seggewiss, R., Lore, K., Guenaga, F. J., Pittaluga, S., Mattapallil, J., Chow, C. K., Koup, R. A., Camphausen, K., Nason, M. C., Meier-Schellersheim, M., Donahue, R. E., Blazar, B. R., Dunbar, C. E., Douek, D. C. (2007). Keratinocyte growth factor augments immune reconstitution after autologous hematopoietic progenitor cell transplantation in rhesus macaques.. Blood 110: 441-449 [Abstract] [Full Text]  
• Abbink, P., Lemckert, A. A. C., Ewald, B. A., Lynch, D. M., Denholtz, M., Smits, S., Holterman, L., Damen, I., Vogels, R., Thorner, A. R., O'Brien, K. L., Carville, A., Mansfield, K. G., Goudsmit, J., Havenga, M. J. E., Barouch, D. H. (2007). Comparative Seroprevalence and Immunogenicity of Six Rare Serotype Recombinant Adenovirus Vaccine Vectors from Subgroups B and D. J. Virol. 81: 4654-4663 [Abstract] [Full Text]  
• Yue, Y., Kaur, A., Eberhardt, M. K., Kassis, N., Zhou, S. S., Tarantal, A. F., Barry, P. A. (2007). Immunogenicity and Protective Efficacy of DNA Vaccines Expressing Rhesus Cytomegalovirus Glycoprotein B, Phosphoprotein 65-2, and Viral Interleukin-10 in Rhesus Macaques. J. Virol. 81: 1095-1109 [Abstract] [Full Text]  
• Sun, Y., Schmitz, J. E., Buzby, A. P., Barker, B. R., Rao, S. S., Xu, L., Yang, Z.-y., Mascola, J. R., Nabel, G. J., Letvin, N. L. (2006). Virus-Specific Cellular Immune Correlates of Survival in Vaccinated Monkeys after Simian Immunodeficiency Virus Challenge. J. Virol. 80: 10950-10956 [Abstract] [Full Text]  
• Leopold, P. L., Wendland, R. L., Vincent, T., Crystal, R. G. (2006). Neutralized Adenovirus-Immune Complexes Can Mediate Effective Gene Transfer via an Fc Receptor-Dependent Infection Pathway.. J. Virol. 80: 10237-10247 [Abstract] [Full Text]  
• Roy, S., Zhi, Y., Kobinger, G. P., Figueredo, J., Calcedo, R., Miller, J. R., Feldmann, H., Wilson, J. M. (2006). Generation of an adenoviral vaccine vector based on simian adenovirus 21. J. Gen. Virol. 87: 2477-2485 [Abstract] [Full Text]  
• Hidmark, A. S., Nordstrom, E. K. L., Dosenovic, P., Forsell, M. N. E., Liljestrom, P., Karlsson Hedestam, G. B. (2006). Humoral Responses against Coimmunized Protein Antigen but Not against Alphavirus-Encoded Antigens Require Alpha/Beta Interferon Signaling. J. Virol. 80: 7100-7110 [Abstract] [Full Text]  
• Letvin, N. L., Mascola, J. R., Sun, Y., Gorgone, D. A., Buzby, A. P., Xu, L., Yang, Z.-y., Chakrabarti, B., Rao, S. S., Schmitz, J. E., Montefiori, D. C., Barker, B. R., Bookstein, F. L., Nabel, G. J. (2006). Preserved CD4+ central memory T cells and survival in vaccinated SIV-challenged monkeys.. Science 312: 1530-1533 [Abstract] [Full Text]  
• Wille-Reece, U., Flynn, B. J., Lore, K., Koup, R. A., Miles, A. P., Saul, A., Kedl, R. M., Mattapallil, J. J., Weiss, W. R., Roederer, M., Seder, R. A. (2006). Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates. J. Exp. Med. 203: 1249-1258 [Abstract] [Full Text]  
• Barratt-Boyes, S. M., Soloff, A. C., Gao, W., Nwanegbo, E., Liu, X., Rajakumar, P. A., Brown, K. N., Robbins, P. D., Murphey-Corb, M., Day, R. D., Gambotto, A. (2006). Broad cellular immunity with robust memory responses to simian immunodeficiency virus following serial vaccination with adenovirus 5- and 35-based vectors. J. Gen. Virol. 87: 139-149 [Abstract] [Full Text]  
• Nanda, A., Lynch, D. M., Goudsmit, J., Lemckert, A. A. C., Ewald, B. A., Sumida, S. M., Truitt, D. M., Abbink, P., Kishko, M. G., Gorgone, D. A., Lifton, M. A., Shen, L., Carville, A., Mansfield, K. G., Havenga, M. J. E., Barouch, D. H. (2005). Immunogenicity of Recombinant Fiber-Chimeric Adenovirus Serotype 35 Vector-Based Vaccines in Mice and Rhesus Monkeys. J. Virol. 79: 14161-14168 [Abstract] [Full Text]