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Journal of Virology, January 2005, p. 87-94, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.87-94.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Improved Enzootic Nasal Tumor Virus Pseudotype Packaging Cell Lines Reveal Virus Entry Requirements in Addition to the Primary Receptor Hyal2

Neal S. Van Hoeven1,2 and A. Dusty Miller1*

Division of Human Biology,1 Molecular and Cellular Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington2

Received 10 June 2004/ Accepted 26 August 2004

Enzootic nasal tumor virus (ENTV) and jaagsiekte sheep retrovirus (JSRV) are closely related retroviruses that cause epithelial cancers of the respiratory tract in sheep and goats. Both viruses use the glycosylphosphatidylinositol (GPI)-anchored cell surface protein hyaluronidase 2 (Hyal2) as a receptor for cell entry, and entry is mediated by the envelope (Env) proteins encoded by these viruses. Retroviral vectors bearing JSRV Env can transduce cells from a wide range of species, with the exception of rodent cells. Because of the low titer of vectors bearing ENTV Env, it has been difficult to determine the tropism of ENTV vectors, which appeared to transduce cells from sheep and humans only. Here we have developed high-titer ENTV packaging cells and confirm that ENTV has a restricted host range compared to that of JSRV. Most cells that are not transduced by JSRV or ENTV vectors can be made susceptible following expression of human Hyal2 on the cells. However, five rat cell lines from different rat strains and different tissues that were engineered to express human Hyal2 were still only poorly infected by ENTV vectors, even though the ENTV Env protein could bind well to human Hyal2 expressed on four of these cell lines. These results indicate the possibility of a coreceptor requirement for these viruses.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Room C2-105, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org.

Journal of Virology, January 2005, p. 87-94, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.87-94.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cote, M., Kucharski, T. J., Liu, S.-L. (2008). Enzootic Nasal Tumor Virus Envelope Requires a Very Acidic pH for Fusion Activation and Infection. J. Virol. 82: 9023-9034 [Abstract] [Full Text]  
  • Coil, D. A., Miller, A. D. (2005). Enhancement of Enveloped Virus Entry by Phosphatidylserine. J. Virol. 79: 11496-11500 [Abstract] [Full Text]  
  • Liu, S.-L., Miller, A. D. (2005). Transformation of Madin-Darby Canine Kidney Epithelial Cells by Sheep Retrovirus Envelope Proteins. J. Virol. 79: 927-933 [Abstract] [Full Text]  


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