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Journal of Virology, January 2005, p. 622-625, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.622-625.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Synchronized Infection of Cell Cultures by Magnetically Controlled Virus

Hillel Haim,1,2* Israel Steiner,2 and Amos Panet1

Department of Virology,1 Laboratory of Neurovirology, Department of Neurology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel2

Received 7 June 2004/ Accepted 27 August 2004

To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.

* Corresponding author. Mailing address: Department of Virology, Hebrew University Faculty of Medicine, Jerusalem 91120, Israel. Phone: 972 2 675 8553. Fax: 972 2 675 7234. E-mail: hhaim{at}md.huji.ac.il.

Journal of Virology, January 2005, p. 622-625, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.622-625.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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