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Journal of Virology, January 2005, p. 592-596, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.592-596.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nonhepatic Cell Lines HeLa and 293 Support Efficient Replication of the Hepatitis C Virus Genotype 2a Subgenomic Replicon

Takanobu Kato,1,2 Tomoko Date,1 Michiko Miyamoto,1 Zijiang Zhao,1,3 Masashi Mizokami,2 and Takaji Wakita1*

Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo,1 Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan,2 Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, People's Republic of China3

Received 17 May 2004/ Accepted 13 August 2004

The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis revealed that mutations in HCV-derived regions were not essential for replication in these cells, as some clones displayed no mutations.

* Corresponding author. Mailing address: Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan. Phone: 81-423-25-3881. Fax: 81-423-21-8678. E-mail: wakita{at}tmin.ac.jp.

Journal of Virology, January 2005, p. 592-596, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.592-596.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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