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Journal of Virology, May 2004, p. 4917-4920, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4917-4920.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Inability of Simian Virus 40 To Establish Productive Infection of Lymphoblastic Cell Lines

Department of Microbiology-Immunology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611

Received 3 September 2003/ Accepted 8 August 2004

Lymphoblastic cell lines were infected with simian virus 40 (SV40) and then monitored for evidence of a productive infection. No evidence of early gene expression was found 2 days following infection, as determined by assaying viral mRNAs and early antigens. Furthermore, only small amounts of virus could be detected by plaque assay 2 days after infection, and levels slowly declined until they were undetectable after a few weeks in culture. Thus, human lymphocytes are not readily infectible with SV40 and do not provide a simple model for studying interactions of SV40 with a human cell type.

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