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Journal of Virology, May 2004, p. 4914-4916, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4914-4916.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector

Jennifer L. Bromberg-White,1 Craig P. Webb,1 Veronique S. Patacsil,2 Cindy K. Miranti,2 Bart O. Williams,3* and Sheri L. Holmen3

Laboratory of Tumor Metastasis and Angiogenesis,1 Laboratory of Integrin Signaling and Tumorigenesis,2 Laboratory of Cell Signaling and Carcinogenesis, Van Andel Research Institute, Grand Rapids, Michigan 495033

Received 21 October 2003/ Accepted 22 December 2003

While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

* Corresponding author. Mailing address: Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503. Phone: (616) 234-5308. Fax: (616) 234-5309. E-mail: bart.williams{at}vai.org.

Journal of Virology, May 2004, p. 4914-4916, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4914-4916.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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