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Journal of Virology, May 2004, p. 4902-4906, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4902-4906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transduction of Terminally Differentiated Neurons by Avian Sarcoma Virus

James G. Greger,1,2 Richard A. Katz,1* Konstantin Taganov,1,3 Glenn F. Rall,1 and Anna Marie Skalka1,2

Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111-2497,1 Graduate Group in Cell and Molecular Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104,2 Department of Molecular Biology and Biotechnology, Russian State Medical University, Moscow, Russian Federation3

Received 8 October 2003/ Accepted 24 December 2003

Recent studies have demonstrated that avian sarcoma virus (ASV) can transduce cycle-arrested cells. Here, we have assessed quantitatively the transduction efficiency of an ASV vector in naturally arrested mouse hippocampal neurons. This efficiency was determined by comparing the number of transduced cells after infection of differentiated neurons versus dividing progenitor cells. The results indicate that ASV is able to transduce these differentiated neurons efficiently and that this activity is not the result of infection of residual dividing cells. The transduction efficiency of the ASV vector was found to be intermediate between the relatively high and low efficiencies obtained with human immunodeficiency virus type 1 and murine leukemia virus vectors, respectively.

* Corresponding author. Mailing address: Fox Chase Cancer Center, Institute for Cancer Research, 333 Cottman Ave., Philadelphia, PA 19111-2497. Phone: (215) 728-3668. Fax: (215) 728-2778. E-mail: r_katz{at}fccc.edu.

Journal of Virology, May 2004, p. 4902-4906, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4902-4906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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