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Journal of Virology, May 2004, p. 4884-4891, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4884-4891.2004

JC Virus Induces Nonapoptotic Cell Death of Human Central Nervous System Progenitor Cell-Derived Astrocytes

Pankaj Seth,¹ Frank Diaz,¹ Jung-Hwa Tao-Cheng,² and Eugene O. Major^1*

Laboratory of Molecular Medicine and Neuroscience,¹ Electron Microscopy Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892²

Received 3 September 2003/ Accepted 23 December 2003

JC virus (JCV), a human neurotropic polyomavirus, demonstrates a selective glial cell tropism that causes cell death through lytic infection. Whether these cells die via apoptosis or necrosis following infection with JCV remains unclear. To investigate the mechanism of virus-induced cell death, we used a human central nervous system progenitor-derived astrocyte cell culture model developed in our laboratory. Using in situ DNA hybridization, immunocytochemistry, electron microscopy, and an RNase protection assay, we observed that astrocytes support a progressive JCV infection, which eventually leads to nonapoptotic cell death. Infected astrocyte cell cultures showed no difference from noninfected cells in mRNA expression of the caspase family genes or in any ultrastructural features associated with apoptosis. Infected cells demonstrated striking necrotic features such as cytoplasmic vacuolization, watery cytoplasm, and dissolution of organelles. Furthermore, staining for caspase-3 and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling were not detected in infected astrocyte cultures. Our findings suggest that JCV-induced cell death of these progenitor cell-derived astrocytes does not utilize an apoptosis pathway but exhibits a pattern of cell destruction consistent with necrotic cell death.

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* Corresponding author. Mailing address: Chief, Laboratory of Molecular Medicine and Neuroscience, NINDS, NIH, Building 36, Room 5W21, 36 Convent Dr., Bethesda, MD 20892-4164. Phone: (301) 496-1635. Fax: (301) 594-5799. E-mail: majorg{at}ninds.nih.gov.

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Journal of Virology, May 2004, p. 4884-4891, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4884-4891.2004

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