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Journal of Virology, May 2004, p. 4591-4598, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4591-4598.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Growth Arrest of Epithelial Cells during Measles Virus Infection Is Caused by Upregulation of Interferon Regulatory Factor 1

Shin-ichi Yokota, Tamaki Okabayashi, Noriko Yokosawa, and Nobuhiro Fujii

Department of Microbiology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8556, Japan

Received 13 November 2003/ Accepted 24 December 2003

Natural infection with measles virus (MeV) is initiated when the virus reaches epithelial cells in the respiratory tract, oropharynx, or conjunctivae. Human epithelial cells infected with MeV frequently show growth suppression. In this study, we investigated the possible mechanisms for this suppression. The bronchiolar epithelial cell A549 showed growth arrest in G0/G1 following MeV infection or treatment with gamma interferon (IFN-{gamma}). IFN regulatory factor-1 (IRF-1) was upregulated during MeV infection, although A549 did not produce IFN-{gamma}. Cells of the cervical squamous cell line SiHa persistently infected with various strains of MeV displayed slower growth than uninfected SiHa cells, although the growth rates varied depending on the MeV strain. Transfection of antisense-oriented IRF-1 cDNA released the MeV-infected SiHa cells from growth suppression. Although these infected cells did not produce IFN-{gamma} and suppressed IFN-{alpha}/ß-induced Jak1 phosphorylation, Jak1 was constitutively phosphorylated. The growth rates negatively correlated with levels of both IRF-1 expression and constitutively phosphorylated Jak1. These results indicate that MeV upregulates IRF-1 in a manner that is independent of IFN but dependent on the JAK/STAT pathway. This induction of IRF-1 appears to suppress cell growth, although the extent seems to vary among MeV strains.

* Corresponding author. Mailing address: Department of Microbiology, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo 060-8556, Hokkaido, Japan. Phone: 81-11-611-2111. Fax: 81-11-612-5861. E-mail: fujii{at}sapmed.ac.jp.

Journal of Virology, May 2004, p. 4591-4598, Vol. 78, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.9.4591-4598.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Okabayashi, T., Yokota, S.-i., Yoto, Y., Tsutsumi, H., Fujii, N. (2009). Fosfomycin Suppresses Chemokine Induction in Airway Epithelial Cells Infected with Respiratory Syncytial Virus. CVI 16: 859-865 [Abstract] [Full Text]  


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