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Journal of Virology, April 2004, p. 3372-3377, Vol. 78, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.5.3372-3377.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Dimerization of Recombinant Tobacco Mosaic Virus Movement Protein

Laurence M. Brill,1,{dagger} Songpon Dechongkit,2 Byron DeLaBarre,3 Jonathon Stroebel,1 Roger N. Beachy,4 and Mark Yeager1,5*

Departments of Cell Biology,1 Chemistry, The Scripps Research Institute,2 Division of Cardiovascular Diseases, Scripps Clinic, La Jolla, California 92037,5 Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305,3 Donald Danforth Plant Science Center, St. Louis, Missouri 631324

Received 16 June 2003/ Accepted 3 November 2003

The p30 movement protein (MP) is essential for cell-to-cell spread of tobacco mosaic virus in planta. We used anion-exchange chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to obtain highly purified 30-kDa MP, which migrated as a single band in native PAGE. Analytical ultracentrifugation suggested that the protein was monodisperse and dimeric in the nonionic detergent n-octyl-ß-D-glucopyranoside. Circular dichroism (CD) spectroscopy showed that the detergent-solubilized protein contained significant {alpha}-helical secondary structure. Proteolysis of the C-tail generated a trypsin-resistant core that was a mixture of primarily monomers and some dimers. We propose that MP dimers are stabilized by electrostatic interactions in the C terminus as well as hydrophobic interactions between putative transmembrane {alpha}-helical coiled coils.

* Corresponding author. Mailing address: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., Mail Drop MB 28, La Jolla, CA 92037. Phone: (858) 784-8584. Fax: (858) 784-2504. E-mail: yeager{at}scripps.edu.

{dagger} Present address: Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121.

Journal of Virology, April 2004, p. 3372-3377, Vol. 78, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.5.3372-3377.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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