This Article Full Text Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Higo-Moriguchi, K. Articles by Taniguchi, K. Search for Related Content PubMed PubMed Citation Articles by Higo-Moriguchi, K. Articles by Taniguchi, K.

Isolation of Human Monoclonal Antibodies That Neutralize Human Rotavirus

Department of Virology and Parasitology,¹ Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan²

Received 10 July 2003/ Accepted 2 December 2003

A human antibody library constructed by utilizing a phage display system was used for the isolation of human antibodies with neutralizing activity specific for human rotavirus. In the library, the Fab form of an antibody fused to truncated cp3 is expressed on the phage surface. Purified virions of strain KU (G1 serotype and P[8] genotype) were used as antigen. Twelve different clones were isolated. Based on their amino acid sequences, they were classified into three groups. Three representative clones—1-2H, 2-3E, and 2-11G—were characterized. Enzyme-linked immunosorbent assay with virus-like particles (VLP-VP2/6 and VLP-VP2/6/7) and recombinant VP4 protein produced from baculovirus recombinants indicated that 1-2H and 2-3E bind to VP4 and that 2-11G binds to VP7. The neutralization epitope recognized by each of the three human antibodies might be human specific, since all of the antigenic mutants resistant to mouse monoclonal neutralizing antibodies previously prepared were neutralized by the human antibodies obtained here. After conversion from the Fab form of an antibody into immunoglobulin G1, the neutralizing activities of these three clones toward various human rotavirus strains were examined. The 1-2H antibody exhibited neutralizing activity toward human rotaviruses with either the P[4] or P[8] genotype. Similarly, the 2-3E antibody showed cross-reactivity against HRVs with the P[6], as well as the P[8] genotype. In contrast, the 2-11G antibody neutralized only human rotaviruses with the G1 serotype. The concentration of antibodies required for 50% neutralization ranged from 0.8 to 20 µg/ml.

This article has been cited by other articles:

• Dong, L., Masaki, Y., Takegami, T., Kawanami, T., Itoh, K., Jin, Z.-X., Huang, C.-R., Tong, X.-P., Fukushima, T., Tanaka, M., Sawaki, T., Sakai, T., Sugai, S., Okazaki, T., Hirose, Y., Umehara, H. (2007). Cloning and expression of two human recombinant monoclonal Fab fragments specific for EBV viral capsid antigen. Int Immunol 19: 331-336 [Abstract] [Full Text]  
• Hunt, A. R., Frederickson, S., Hinkel, C., Bowdish, K. S., Roehrig, J. T. (2006). A humanized murine monoclonal antibody protects mice either before or after challenge with virulent Venezuelan equine encephalomyelitis virus. J. Gen. Virol. 87: 2467-2476 [Abstract] [Full Text]  
• Kakita, M., Takahashi, T., Komiya, T., Iba, Y., Tsuji, T., Kurosawa, Y., Takahashi, M. (2006). Isolation of a Human Monoclonal Antibody with Strong Neutralizing Activity against Diphtheria Toxin.. Infect. Immun. 74: 3682-3683 [Abstract] [Full Text]  
• Monnier, N., Higo-Moriguchi, K., Sun, Z.-Y. J., Prasad, B. V. V., Taniguchi, K., Dormitzer, P. R. (2006). High-Resolution Molecular and Antigen Structure of the VP8* Core of a Sialic Acid-Independent Human Rotavirus Strain. J. Virol. 80: 1513-1523 [Abstract] [Full Text]