This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harrington, P. R.
Right arrow Articles by Baric, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harrington, P. R.
Right arrow Articles by Baric, R. S.

 Previous Article  |  Next Article 

Journal of Virology, March 2004, p. 3035-3045, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3035-3045.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Norovirus Capture with Histo-Blood Group Antigens Reveals Novel Virus-Ligand Interactions

Patrick R. Harrington,1,{dagger} Jan Vinjé,2 Christine L. Moe,3 and Ralph S. Baric1,4*

Department of Microbiology and Immunology, School of Medicine,1 Department of Environmental Sciences and Engineering,2 Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599,4 Department of International Health, Rollins School of Public Health, Emory University, Atlanta, Georgia 303223

Received 9 September 2003/ Accepted 18 November 2003

Noroviruses are genetically diverse, uncultivable, positive-sense RNA viruses and are the most common cause of epidemic acute gastroenteritis in humans in the United States. Recent studies of norovirus attachment in vitro by using recombinant virus-like particles (VLPs) suggest that various norovirus strains exhibit different patterns of attachment to ABH histo-blood group antigens, which are carbohydrate epitopes present in high concentrations on mucosal cell surfaces of the gut. However, attachment of live norovirus strains to histo-blood group antigens has not been investigated to date. Utilizing a newly designed magnetic bead-virus capture method, we characterized histo-blood group antigen attachment properties of various norovirus strains obtained from clinical stool specimens to compare the attachment properties of wild-type virus and VLPs and to further map norovirus attachment. Consistent with previous reports using VLPs, various strains of noroviruses exhibited different patterns of attachment to histo- blood group antigens. Norwalk virus bound specifically to H type 1, H type 3, and Leb. Two genogroup II noroviruses, one representing the Toronto genotype and the other from a novel genotype, bound specifically to Leb. A Desert Shield-like strain did not attach to H types 1, 2, or 3, H type 1 and 3 precursors, Lea, or Leb. Surprisingly, wild-type Snow Mountain virus (SMV) attached specifically to H type 3, which contradicted previous findings with SMV VLPs. On further investigation, we found that stool components promote this attachment, providing the first known observation that one or more components of human feces could promote and enhance norovirus attachment to histo-blood group antigens.

* Corresponding author. Mailing address: Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7435. Phone: (919) 966-3895. Fax: (919) 966-2089. E-mail: rbaric{at}sph.unc.edu.

{dagger} Present address: Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295.

Journal of Virology, March 2004, p. 3035-3045, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3035-3045.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Morton, V., Jean, J., Farber, J., Mattison, K. (2009). Detection of Noroviruses in Ready-To-Eat Foods by Using Carbohydrate-Coated Magnetic Beads. Appl. Environ. Microbiol. 75: 4641-4643 [Abstract] [Full Text]  
  • Cannon, J. L., Lindesmith, L. C., Donaldson, E. F., Saxe, L., Baric, R. S., Vinje, J. (2009). Herd Immunity to GII.4 Noroviruses Is Supported by Outbreak Patient Sera. J. Virol. 83: 5363-5374 [Abstract] [Full Text]  
  • Rydell, G. E, Nilsson, J., Rodriguez-Diaz, J., Ruvoen-Clouet, N., Svensson, L., Le Pendu, J., Larson, G. (2009). Human noroviruses recognize sialyl Lewis x neoglycoprotein. Glycobiology 19: 309-320 [Abstract] [Full Text]  
  • Rodriguez, R. A., Pepper, I. L., Gerba, C. P. (2009). Application of PCR-Based Methods To Assess the Infectivity of Enteric Viruses in Environmental Samples. Appl. Environ. Microbiol. 75: 297-307 [Full Text]  
  • Cannon, J. L., Vinje, J. (2008). Histo-Blood Group Antigen Assay for Detecting Noroviruses in Water. Appl. Environ. Microbiol. 74: 6818-6819 [Abstract] [Full Text]  
  • Shirato, H., Ogawa, S., Ito, H., Sato, T., Kameyama, A., Narimatsu, H., Xiaofan, Z., Miyamura, T., Wakita, T., Ishii, K., Takeda, N. (2008). Noroviruses Distinguish between Type 1 and Type 2 Histo-Blood Group Antigens for Binding. J. Virol. 82: 10756-10767 [Abstract] [Full Text]  
  • Choi, J.-M., Hutson, A. M., Estes, M. K., Prasad, B. V. V. (2008). Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus. Proc. Natl. Acad. Sci. USA 105: 9175-9180 [Abstract] [Full Text]  
  • Siebenga, J. J., Vennema, H., Renckens, B., de Bruin, E., van der Veer, B., Siezen, R. J., Koopmans, M. (2007). Epochal Evolution of GGII.4 Norovirus Capsid Proteins from 1995 to 2006. J. Virol. 81: 9932-9941 [Abstract] [Full Text]  
  • Cheetham, S., Souza, M., McGregor, R., Meulia, T., Wang, Q., Saif, L. J. (2007). Binding Patterns of Human Norovirus-Like Particles to Buccal and Intestinal Tissues of Gnotobiotic Pigs in Relation to A/H Histo-Blood Group Antigen Expression. J. Virol. 81: 3535-3544 [Abstract] [Full Text]  
  • Feng, X., Jiang, X. (2007). Library Screen for Inhibitors Targeting Norovirus Binding to Histo-Blood Group Antigen Receptors. Antimicrob. Agents Chemother. 51: 324-331 [Abstract] [Full Text]  
  • Tan, M., Meller, J., Jiang, X. (2006). C-terminal arginine cluster is essential for receptor binding of norovirus capsid protein.. J. Virol. 80: 7322-7331 [Abstract] [Full Text]  
  • Holgersson, J., Lofling, J. (2006). Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity. Glycobiology 16: 584-593 [Abstract] [Full Text]  
  • Costantini, V., Loisy, F., Joens, L., Le Guyader, F. S., Saif, L. J. (2006). Human and animal enteric caliciviruses in oysters from different coastal regions of the United States.. Appl. Environ. Microbiol. 72: 1800-1809 [Abstract] [Full Text]  
  • Thorven, M., Grahn, A., Hedlund, K.-O., Johansson, H., Wahlfrid, C., Larson, G., Svensson, L. (2005). A Homozygous Nonsense Mutation (428G->A) in the Human Secretor (FUT2) Gene Provides Resistance to Symptomatic Norovirus (GGII) Infections. J. Virol. 79: 15351-15355 [Abstract] [Full Text]  
  • Tan, M., Jiang, X. (2005). The P Domain of Norovirus Capsid Protein Forms a Subviral Particle That Binds to Histo-Blood Group Antigen Receptors. J. Virol. 79: 14017-14030 [Abstract] [Full Text]  
  • Lochridge, V. P., Jutila, K. L., Graff, J. W., Hardy, M. E. (2005). Epitopes in the P2 domain of norovirus VP1 recognized by monoclonal antibodies that block cell interactions. J. Gen. Virol. 86: 2799-2806 [Abstract] [Full Text]  
  • Huang, P., Farkas, T., Zhong, W., Tan, M., Thornton, S., Morrow, A. L., Jiang, X. (2005). Norovirus and Histo-Blood Group Antigens: Demonstration of a Wide Spectrum of Strain Specificities and Classification of Two Major Binding Groups among Multiple Binding Patterns. J. Virol. 79: 6714-6722 [Abstract] [Full Text]  
  • Farkas, T., Nakajima, S., Sugieda, M., Deng, X., Zhong, W., Jiang, X. (2005). Seroprevalence of Noroviruses in Swine. J. Clin. Microbiol. 43: 657-661 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»